The influence of the sample preparation on plasma protein adsorption patterns on polysaccharide-stabilized iron oxide particles and N-terminal microsequencing of unknown proteins

Journal of Drug Targeting
K ThodeMayk Kresse

Abstract

The in vivo organ distribution of i.v. injected drug carriers is strongly influenced by the adsorption of plasma proteins after i.v. injection, e.g. uptake by the mononuclear phagocytic system (MPS). 2-D PAGE could be established to analyze plasma protein adsorption patterns on polysaccharide-stabilized aqueous iron oxide dispersions used as contrast agents in Magnetic Resonance Imaging (MRI). After incubation in human plasma, centrifugation, a washing procedure and a solubilization step were carried out to obtain the proteins adsorbed onto these ultrasmall particles (65 nm in diameter). Patterns of adsorbed proteins were analyzed in dependence on the washing medium used, i.e. highly purified water, phosphate buffered saline and Krebs buffer pH 7.4. Conductivity and composition of the washing medium influenced the adsorption of IgG onto the particles, but had little effect on the other proteins present. IgG was strongly reduced when using the relatively high conductive buffers. The more stabilizing polysaccharide was desorbed the larger was the total amount of adsorbed proteins. Appearance of two unknown chains of spots in the range of appr. 92 kDa, accounting for appr. 10% and 2% of the overall detected protein amount, was obs...Continue Reading

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Citations

Feb 13, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·K S SoppimathW E Rudzinski
Oct 13, 2007·International Journal of Pharmaceutics·Aiping ZhuTianqing Liao
Aug 16, 2015·Biomaterials·Lisa LandgrafIngrid Hilger
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Rashmi Gupta, A K Bajpai

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