PMID: 2499455Jun 1, 1989Paper

The influence of tryptophan on mobility of residues in the trp repressor of Escherichia coli

European Journal of Biochemistry
A N Lane

Abstract

The relative mobility of residues in the trp repressor of Escherichia coli has been examined in the absence and presence of the corepressor L-tryptophan by one- and two-dimensional 1H NMR. A comparison of relative intensities of cross peaks in NOESY and COSY spectra allowed a rigid Tyr and a mobile Tyr residue, three mobile Ser residues and three mobile Lys residues to be detected. The two Tyr residues were assigned by selective nitration with tetranitromethane. The singly nitrated molecule (on Tyr7) binds the trp operator with an affinity close to that of the unmodified repressor. Measurements of the intraring cross-relaxation rate constant as a function of temperature for Tyr7 shows the presence of considerable internal motion on the subnanosecond time scale in the flexible N-terminal arm. The order parameter, S2, characterising the motion is 0.35, which increases to about 0.5 in the presence of Trp. Trp decreases both the amplitude of the motion and the rate of the motion. At least three of the six Ser residues of the trp repressor have greater mobility than expected for a rigid body, and two of the Ser residues are sensitive to the presence of Trp. The more mobile Ser residues are probably those on the N-terminal arm and th...Continue Reading

References

Nov 17, 1987·Biochemistry·P Leighton, P Lu
Oct 15, 1985·European Journal of Biochemistry·A N Lane, O Jardetzky
May 27, 1986·Biochimica Et Biophysica Acta·A N LaneO Jardetzky
May 1, 1987·Journal of Bacteriology·C L Grove, R P Gunsalus
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S SearleA J Geddes
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Carey
Mar 15, 1988·The Biochemical Journal·L R Chandler, A N Lane
Aug 11, 1987·Biochemistry·J F LefèvreO Jardetzky
Jan 1, 1988·Proteins·C L LawsonP B Sigler
Oct 24, 1986·Nucleic Acids Research·J L Paluh, C Yanofsky
Jun 6, 1987·Biochimica Et Biophysica Acta·A N LaneO Jardetzky
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R L Kelley, C Yanofsky
Oct 6, 1985·Nature·R W SchevitzP B Sigler
Jan 1, 1984·CRC Critical Reviews in Biochemistry·D R Kearns
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A JoachimiakP B Sigler
Oct 23, 1984·Biochemistry·M A WeissM Karplus
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R P Gunsalus, C Yanofsky
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R L Kelley, C Yanofsky
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee

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Citations

Oct 22, 2005·The British Journal of Educational Psychology·Ton Mooij
Sep 7, 2006·The British Journal of Educational Psychology·Paul NaylorFrancesca Lemme
Nov 20, 1992·Biochimica Et Biophysica Acta·J N EvansM F Roberts

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