PMID: 3056721Nov 1, 1988

The influence of water on protease-catalyzed peptide synthesis in acetonitrile/water mixtures

European Journal of Biochemistry
M ReslowB Mattiasson

Abstract

Protease-catalyzed peptide synthesis in acetonitrile/water mixtures, containing 0-90% water, was investigated. alpha-Chymotrypsin, as well as thermolysin, were deposited on solid supports, prior to exposure to the reaction media. Peptide syntheses were performed using both a kinetically controlled process (chymotrypsin) and an equilibrium-controlled synthesis (thermolysin). The activity of chymotrypsin decreased at low water contents. However, at low water contents (1-10%) hydrolytic side reactions were suppressed and high yields of dipeptides were obtained. Optimal water content for the thermolysin-catalyzed reaction was 4-8%. The dipeptides produced were fully soluble in the reaction mixtures. High operational stability for alpha-chymotrypsin was obtained during 216 h of reaction.

References

Oct 1, 1977·Journal of Biochemistry·T Oka, K Morihara
Jun 1, 1977·The Biochemical Journal·K Morihara, T Oka
Mar 1, 1975·Analytical Biochemistry·S L Snyder, P Z Sobocinski
Sep 22, 1971·Biochimica Et Biophysica Acta·P J RobinsonM D Lilly
Jan 13, 1984·Biochemical and Biophysical Research Communications·J D Finkelstein, J J Martin
Jan 13, 1984·Biochemical and Biophysical Research Communications·D D Petkov, I Stoineva
May 1, 1984·International Journal of Peptide and Protein Research· Mitin YuV Gorbunova EYu
Sep 1, 1980·Journal of Biochemistry·T Oka, K Morihara

Citations

Apr 1, 1999·Biotechnology and Bioengineering·M ErbeldingerP J Halling
May 9, 2000·Biotechnology and Bioengineering·S J Novick, J S Dordick
Jan 20, 1993·Biotechnology and Bioengineering·E WehtjeB Mattiasson
Jul 1, 1992·Enzyme and Microbial Technology·P ClapésP Adlercreutz
Feb 15, 1996·Enzyme and Microbial Technology·I GillE N Vulfson
Jan 15, 1992·European Journal of Biochemistry·M N Gupta
Jan 1, 1990·Annals of the New York Academy of Sciences·P AdlercreutzB Mattiasson
Apr 9, 2008·Journal of Oleo Science·Sharifah MohamadMohd Zaki Abd Rahman
May 24, 2011·Enzyme Research·Ashok Kumar, Shamsher Singh Kanwar
Sep 6, 2001·Biotechnology and Bioengineering·J A Laszlo, D L Compton
Apr 15, 2016·Microbiology and Molecular Biology Reviews : MMBR·John E Cronan
Aug 13, 2008·Bioorganicheskaia khimiia·A V BeliaevaI Iu Filippova

Related Concepts

Acetonitrile, 3H-labeled
Acetonitriles
Adsorption
Catalysis
Avazyme
Dipeptides
Drug Stability
Hydrolysis
Peptide Hydrolases
Polypeptides

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