PMID: 8995663Feb 1, 1997Paper

The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA

Journal of Virology
B Perales, J Ortín

Abstract

The transcription and replication of influenza virus RNA (vRNA) were reconstituted in vivo. The experimental approach involved the transfection of plasmids encoding the viral subunits of the polymerase and the nucleoprotein into cells infected with a vaccinia virus recombinant virus expressing the T7 RNA polymerase. As templates, one of two model RNAs was transfected: vNSZ or cNSZ RNA. The RNAs were 240 nucleotides in length, contained the terminal sequences of the NS viral segment, and were of negative or positive polarity, respectively. The accumulation of cRNA and mRNA in cells transfected with vNSZ RNA and the accumulation of vRNA and mRNA in cells transfected with cNSZ RNA were determined by RNase protection assays with labeled vNSZ-L or cNSZ-L probes. The patterns of protected bands obtained indicated that both cRNA replication intermediate and mRNA accumulated when the system was reconstituted with vNSZ RNA. Likewise, both vRNA and mRNA accumulated after reconstitution with cNSZ RNA. The reconstitution of incomplete systems in which any of the subunits of the polymerase or the model RNA were omitted was completely negative for the accumulation of cRNA or vRNA, indicating that the presence of the PB2 subunit in the polyme...Continue Reading

References

Jun 1, 1992·Virus Research·A NietoJ Ortín
Jun 1, 1991·The Journal of General Virology·J ValcárcelJ Ortín
Nov 1, 1990·Journal of Virology·T S HuangM Krystal
Apr 1, 1990·Journal of Biochemistry·A HondaA Ishihama
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M T HsuP Palese
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A R Beaton, R M Krug
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I UlmanenR M Krug
Jan 30, 1982·Virology·A J HayJ McCauley
Aug 11, 1982·Nucleic Acids Research·D BlaasE Kuechler
Apr 1, 1981·Journal of Virology·J S RobertsonR A Lazzarini
May 1, 1993·Virus Research·M E PicconeP Palese
Jul 1, 1993·The Journal of General Virology·J ValcárcelJ Ortín
Jan 1, 1996·Archives of Virology·M KobayashiA Ishihama

❮ Previous
Next ❯

Citations

Dec 23, 2004·Journal of Virology·Atsushi KawaguchiKyosuke Nagata
Apr 3, 2008·Reviews in Medical Virology·Kyosuke NagataTadasuke Naito
Jun 27, 2009·PLoS Pathogens·Rocío ColomaJaime Martín-Benito
Aug 13, 2010·Journal of Virology·Patricia Resa-InfanteJuan Ortín
Mar 2, 2011·RNA Biology·Patricia Resa-InfanteJuan Ortin
Jun 20, 2002·Journal of Virology·George G Brownlee, Jane L Sharps
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Estela AreaJuan Ortín
Dec 25, 2010·The Journal of Biological Chemistry·Benjamin MänzMartin Schwemmle
May 2, 2007·Annals of the New York Academy of Sciences·Timothy K W Cheung, Leo L M Poon
May 28, 2009·Science in China. Series C, Life Sciences·Yingfang LiuZihe Rao
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ayae HondaAkira Ishihama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.