The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins

The EMBO Journal
R E O'NeillPeter Palese

Abstract

Nuclear import and export of viral nucleic acids is crucial for the replication cycle of many viruses, and elucidation of the mechanism of these steps may provide a paradigm for understanding general biological processes. Influenza virus replicates its RNA genome in the nucleus of infected cells. The influenza virus NS2 protein, which had no previously assigned function, was shown to mediate the nuclear export of virion RNAs by acting as an adaptor between viral ribonucleoprotein complexes and the nuclear export machinery of the cell. A functional domain on the NS2 with characteristics of a nuclear export signal was mapped: it interacts with cellular nucleoporins, can functionally replace the effector domain of the human immunodeficiency virus type 1 (HIV-1) Rev protein and mediates rapid nuclear export when cross-linked to a reporter protein. Microinjection of anti-NS2 antibodies into infected cells inhibited nuclear export of viral ribonucleoproteins, suggesting that the Rev-like NS2 mediates this process. Therefore, we have renamed this Rev-like factor the influenza virus nuclear export protein or NEP. We propose a model by which NEP acts as a protein adaptor molecule bridging viral ribonucleoproteins and the nuclear pore co...Continue Reading

References

Nov 1, 1978·Virology·R A LambP W Choppin
Feb 20, 1992·Nature·S Piñol-Roma, G Dreyfuss
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T J HopeT G Parslow
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B K FelberG N Pavlakis
Jan 1, 1995·Archives of Virology·A C WardI G Macreadie
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Jan 1, 1995·Annual Review of Biochemistry·L I Davis
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A FridellB R Cullen
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A FridellB R Cullen

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Citations

Jan 12, 2002·Reviews in Medical Virology·Gabriele Neumann, Yoshihiro Kawaoka
Jun 6, 2009·Archives of Virology·Chenfei ZhangKun Qian
Oct 20, 2012·Bulletin of Mathematical Biology·Alex MadrahimovJim Rogers
Oct 24, 2002·Journal of Virological Methods·B C E P D OliveiraM C Cabral
May 30, 2002·Virus Research·Olga P ZouevaEarl G Brown
Jun 29, 2000·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·E G Brown
Aug 6, 2002·Current Opinion in Microbiology·Jonathan Yewdell, Adolfo García-Sastre
Jul 17, 2001·RNA·S TchatalbachevG Hobom
May 14, 2010·Animal Health Research Reviews·Wenjun Ma, Jürgen A Richt
Jul 2, 2010·Animal Health Research Reviews·Heather L Forrest, Robert G Webster
Dec 21, 2012·Animal Health Research Reviews·Qi ChenD L Hank Harris
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·David JacksonRobert A Lamb
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew VarbleBenjamin R tenOever
Oct 26, 1999·Viral Immunology·S LudwigT Wolff
Oct 25, 2012·Nucleic Acids Research·Szu-Chin FuHsueh-Fen Juan
Jan 10, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J K TaubenbergerT G Fanning
Jan 9, 2001·Clinical Microbiology Reviews·T Horimoto, Y Kawaoka
Apr 29, 2011·Journal of Virology·Amie J EisfeldYoshihiro Kawaoka
Aug 3, 2012·Journal of Virology·Atsushi KawaguchiKyosuke Nagata
Jun 4, 2010·Journal of Virology·Qinshan GaoPeter Palese
May 31, 2013·Journal of Virology·Steven F BakerLuis Martínez-Sobrido
Oct 5, 2012·Journal of Virology·Jasmine T PerezBenjamin R tenOever
Feb 20, 2009·Journal of Virology·Zejun LiYoshihiro Kawaoka

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