The inhibition of gluconeogenesis by gatifloxacin may contribute to its hypoglycaemic action

European Journal of Pharmacology
Jakub DrozakJadwiga Bryla

Abstract

The action of gatifloxacin, the broad-spectrum fluoroquinolone antibiotic commonly used in the therapy of various bacterial infections, was investigated in isolated rabbit hepatocytes and kidney-cortex tubules by measuring the activity of gluconeogenesis, a process that maintains whole body glucose homeostasis. The data show that in kidney-cortex tubules, application of gatifloxacin at up to 100 microM was followed by a marked accumulation of the drug in the intracellular milieu and a decrease in the rate of glucose formation from pyruvate by 20-50%. Gatifloxacin did not affect the rate of gluconeogenesis from either alanine + glycerol + octanoate or aspartate + glycerol + octanoate. At concentrations between 25 and 200 microM the drug decreased mitochondrial oxygen consumption by 20-45% with pyruvate + malate and ADP. As in the case of alpha-cyano-4-hydroxycinnamate, a well-established inhibitor of the mitochondrial pyruvate transporter, it diminished pyruvate uptake by both renal and hepatic mitochondria. The inhibitory action of gatifloxacin was less pronounced in hepatocytes where reduction in pyruvate-dependent glucose formation and mitochondrial respiration was by no more than 25%. The antibiotic did not influence mitocho...Continue Reading

References

Nov 1, 1992·Kidney International·H J Adrogué
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J R WilliamsonP W Felts
Nov 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Van den Bossche
Jan 1, 1982·The International Journal of Biochemistry·J ZaleskiJ Bryla
Sep 13, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D M Grasela
Feb 24, 2001·Pharmacotherapy·D N Fish, D S North
Feb 24, 2001·Diabetes Care·J E GerichM Stumvoll
Jun 19, 2002·The Journal of Biological Chemistry·Jonathan V RocheleauDavid W Piston
Jul 6, 2005·Toxicology and Applied Pharmacology·Kevin ParkMunir Pirmohamed
Nov 15, 2005·The American Journal of the Medical Sciences·Romi BhasinRosemarie Pasmantier
Nov 29, 2005·Journal of Pharmaceutical and Biomedical Analysis·Saleh Al-DgitherMuhammad M Hammami
Mar 2, 2006·Biological & Pharmaceutical Bulletin·Yasuyoshi IshiwataMasato Yasuhara
Mar 24, 2006·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Claire Kendall, Eric Wooltorton
Oct 31, 2006·European Journal of Pharmacology·Chizumi YamadaNobuya Inagaki
Dec 1, 2006·Chemico-biological Interactions·María José Gómez-LechónMaría Teresa Donato
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Takashi TomitaKenji Kihira
Aug 31, 2007·European Journal of Pharmacology·Tian-Fang GeYuan-Yuan Ho

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Citations

Nov 29, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hany GhalyIngo Rustenbeck
Mar 10, 2015·The Biochemical Journal·Kyle S McCommis, Brian N Finck
Dec 2, 2011·The Journal of Toxicological Sciences·Kesavan K SureshMahesh J Kulkarni
Feb 1, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Takuma IguchiKazuhiko Mori

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