PMID: 11916110Mar 28, 2002Paper

The inhibition of ice nucleators by insect antifreeze proteins is enhanced by glycerol and citrate

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
J G Duman

Abstract

Antifreeze proteins depress the freezing point of water while not affecting the melting point, producing a characteristic difference in freezing and melting points termed thermal hysteresis. Larvae of the beetle Dendroides canadensis accumulate potent antifreeze proteins (DAFPs) in their hemolymph and gut, but to achieve high levels of thermal hysteresis requires enhancers, such as glycerol. DAFPs have previously been shown to inhibit the activity of bacterial and hemolymph protein ice nucleators, however, the effect was not large and therefore the effectiveness of the DAFPs in promoting supercooling of the larvae in winter was doubtful. However, this study demonstrates that DAFPs, in combination with the thermal hysteresis enhancers glycerol (1 M) or citrate (0.5 M), eliminated the activity of hemolymph protein ice nucleators and Pseudomonas syringae ice-nucleating active bacteria, and lowered the supercooling points (nucleation temperatures) of aqueous solutions containing these ice nucleators to those of water or buffer alone. This shows that the DAFPs, along with glycerol, play a critical role in promoting hemolymph supercooling in overwintering D. canadensis. Also, DAFPs in combination with enhancers may be useful in appli...Continue Reading

Citations

Nov 6, 2003·Phytochemistry·Okkeş Atici, Barbaros Nalbantoglu
Jan 21, 2016·Biointerphases·Anneloes S Oude VrielinkIlja K Voets
Sep 25, 2012·Journal of Insect Physiology·Erlend KristiansenHans Ramløv
Mar 13, 2012·Cryobiology·Chikako KuwabaraSeizo Fujikawa
Aug 19, 2009·Journal of Insect Physiology·Aleksandra Walczyńska
Jul 16, 2008·Biochimica Et Biophysica Acta·Natapol AmornwittawatXin Wen
May 20, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Jon P CostanzoGordon R Ultsch
Jul 11, 2006·Journal of Insect Physiology·Jessie NicodemusJohn G Duman
Jul 5, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E KristiansenK E Zachariassen
May 7, 2013·Cryobiology·Chikako KuwabaraSeizo Fujikawa
May 6, 2014·Cryobiology·Chikako KuwabaraSeizo Fujikawa
Sep 26, 2017·Bioscience, Biotechnology, and Biochemistry·Shingo SuzukiKeita Arakawa
Aug 5, 2017·The Journal of Experimental Biology·Henry M Vu, John G Duman
Mar 7, 2008·Critical Reviews in Biotechnology·S Venketesh, C Dayananda
Mar 12, 2020·International Journal of Biological Macromolecules·Hong XiangYong Hu
Oct 14, 2008·Biomacromolecules·Kunio FunakoshiTakashi Tomita
Jan 26, 2013·The Journal of Experimental Biology·Philip K NickellJohn G Duman

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