The inhibition of inducible nitric oxide production in lipopolysaccharide-stimulated rat macrophages and in silico studies by flavonoids from Iris spuria L. rhizomes.

Journal of Ethnopharmacology
Iman S A KhallafSalwa F Farag

Abstract

Iris is the largest genus in the family Iridaceae. Iris plants are distributed in tropical regions of the world. They are used as ornamentals and traditionally used to treat a variety of ailments. This study aimed to evaluate the anti-inflammatory effect of flavonoids isolated from Iris spuria L. The isolated flavonoids (1-4) were identified on the basis of different spectroscopic methods (1D- and 2D-NMR) and co-TLC with authentic samples. The anti-inflammatory effect was tested on lipopolysaccharide (LPS)-induced nitric oxide (NO) production from rat-isolated peritoneal macrophages. Modeling and docking simulations of the compounds were performed using Molecular Operating Environment software and the crystal structure of the murine inducible nitric oxide synthase (iNOS). Four flavonoids (1-4) had been isolated from the rhizomes of Iris spuria L. (Hocka Hoona) for the first time. They were characterized as 5,7,2'-trihydroxy-6-methoxyflavanone (1), tectorigenin 7-O-β-D-glucopyranoside (2), tectorigenin 4'-O-β-D-glucopyranoside (3), and tectorigenin 4'-O-[β-D-glucopyranosyl(1 → 6)-β-D-glucopyranoside] (4). The selective inducible NO synthase inhibitor; aminoguanidine was used as a positive control. The production of nitric oxide ...Continue Reading

References

Feb 17, 2001·Nitric Oxide : Biology and Chemistry·K M MirandaD A Wink
May 29, 2002·Phytochemistry·Franz-Josef MarnerMathias Schäfer
May 10, 2003·Journal of Ethnopharmacology·Atta-ur RahmanM Iqbal Choudhary
Nov 24, 2004·Archives of Pharmacal Research·Abdel Nasser B Singab
Apr 14, 2006·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Abdel Nasser B SingabKalevi Pihlaja
Dec 7, 2006·American Journal of Pharmaceutical Education·Blase Billack
May 13, 2008·Phytochemistry·Muhammad Iqbal ChoudharyMudassir Israr Zaidi
Sep 16, 2008·Journal of Natural Medicines·Salwa F FaragTsutomu Hatano
Nov 20, 2008·Current Protocols in Immunology·Xia ZhangDavid M Mosser
Mar 15, 2011·Bioorganic & Medicinal Chemistry Letters·David R CheshireAlan V Wallace
Mar 14, 2011·Molecular Informatics·Alexey LaguninVladimir Poroikov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.