The inhibition of invasion of human melanoma cells through N-cadherin knock-down

Medical Oncology
Dorota Ciołczyk-Wierzbicka, Piotr Laidler

Abstract

N-cadherin seems to promote cell migration and invasion in many types of cancers. The object of this study is recognition of the possible role of N-cadherin and selected downstream protein kinases: PI3K, ERK1/2, and mTOR in cell invasion in malignant melanoma. Melanoma cells were transfected with the small interfering RNA (siRNA) that targets human N-cadherin gene (CDH2). Inhibitors LY294002 (PI3K), U0126 (ERK1/2), and everolimus (mTOR) were used to inhibit selected kinases of signalling pathways. In vitro cell invasion was studied using Matrigel and an analysis of matrix metalloproteinases MMP-2 and MMP-9 activity by gelatinase zymogram assay. Treatment of melanoma cell with either siRNA against N-cadherin or protein kinase inhibitors led to significantly decreased MMPs expression and activity, as well as diminished invasion. Both the current and the former results suggest that activation of PI3/AKT, mTOR, and ERK kinase, following N-cadherin expression, contributes not only to increased proliferation but also invasive potential of melanoma cells. The results also indicate that N-cadherin, as well as the studied kinases, should be considered as a potential target in melanoma therapy.

References

Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S Steinberg, M Takeichi
May 22, 2004·Annals of the New York Academy of Sciences·Rachel B HazanKimita Suyama
Sep 7, 2004·The International Journal of Developmental Biology·Lara D M Derycke, Marc E Bracke
Sep 29, 2006·The Journal of Biological Chemistry·William I Weis, W James Nelson
Mar 7, 2008·Journal of Cell Science·Margaret J WheelockKeith R Johnson
May 16, 2008·Nature·Matthieu CaveyThomas Lecuit
May 13, 2010·Molecular Cancer Research : MCR·Mahmut Yilmaz, Gerhard Christofori
Jul 16, 2010·Dermatology Research and Practice·R RiaA Vacca
Mar 30, 2011·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Koen JacobsMarc Bracke
Jul 19, 2011·Trends in Cell Biology·Guillermo A GomezAlpha S Yap
Sep 6, 2011·Current Opinion in Cell Biology·Deborah E LeckbandJohan de Rooij
Jan 21, 2012·Cancer Letters·Yekaterina Y ZaytsevaB Mark Evers
Mar 1, 2012·Pathology International·Takahito KamikiharaShoji Natsugoe
Aug 7, 2012·Bulletin of Experimental Biology and Medicine·M B AksenenkoT G Ruksha
Feb 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Federica CatalanottiPaul B Chapman
May 10, 2013·Bulletin of Experimental Biology and Medicine·M B Aksenenko, T G Ruksha
Sep 27, 2013·Frontiers in Oncology·Danielle E DyeDeirdre R Coombe
Apr 3, 2014·The Journal of Biological Chemistry·Sabine PokuttaWilliam I Weis
Sep 2, 2014·International Journal of Oncology·Andrew WalkerKathryn R Lawson
Oct 30, 2015·British Journal of Cancer·Carole SiretFrédéric André
Nov 23, 2015·Seminars in Cancer Biology·Keith I BlockMassimo Zollo
Sep 30, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dorota GilPiotr Laidler

❮ Previous
Next ❯

Citations

Aug 4, 2018·Medical Oncology·Qinghong KongGuanlin Wang
Mar 9, 2019·Journal of Cell Communication and Signaling·Dorota Ciołczyk-WierzbickaPiotr Laidler
Oct 6, 2019·Human Cell·Dorota Ciołczyk-WierzbickaPiotr Laidler
Oct 21, 2020·International Journal of Molecular Sciences·Ilona KaszakPiotr Jurka
Aug 28, 2021·Biomolecules·Cian D'Arcy, Christina Kiel

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
transfection
in vitro transcription
reverse transcription-PCR
zymography
electrophoresis
biopsies

Software Mentioned

Statistica
SynGene Gene Tools
StatSoft

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.