Abstract
In the present study, the role of microRNA‑15a (miR‑15a) was investigated in hepatitis B virus (HBV)‑associated liver cancer. The results revealed that the expression levels of miR-15a were increased in HBV-associated liver cancer tissues compared with the levels in normal tumor‑adjacent tissues. Moreover, Smad-7 protein expression in patients with HBV-associated liver cancer was higher than that in normal tumor-adjacent tissues. In addition, miR-15a expression and Smad-7 protein expression were increased in HepG2 hepatocellular carcinoma cells compared with that noted in L-02 normal hepatocytes. In HepG2 cells, miR-15a inhibition suppressed cell proliferation and increased Smad-7 protein expression. The inhibition of miR-15a was also demonstrated to decrease transforming growth factor (TGF)-β1 protein expression and Smad-2, p-Smad-2 and Smad-4 expression levels in HepG2 cells. Furthermore, FSP1 protein expression and caspase-3/-7 activities were enhanced by miR-15a inhibition in HepG2 cells compared with the control group. Treatment with recombinant TGF-β1 was demonstrated to activate Smad‑2/-4 and FSP1 protein expression and increase caspase-3/-7 activity in HepG2 cells. Collectively, these findings demonstrate that the miR-1...Continue Reading
References
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K MatsuzakiK Inoue
Dec 12, 2002·American Journal of Medical Genetics. Part a·M Michael Cohen
Apr 7, 2006·Journal of Cellular Biochemistry·Yibin Kang
Feb 25, 2009·Current Opinion in Cell Biology·Carl-Henrik HeldinAristidis Moustakas
Dec 15, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Edoardo G GianniniUNKNOWN Italian Liver Cancer (ITA.LI.CA.) group
Aug 14, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Jinxin ZhengCalvin Q Pan
Mar 28, 2013·International Journal of Molecular Sciences·Andrea ConidiDanny Huylebroeck
Jul 25, 2013·Critical Reviews in Eukaryotic Gene Expression·Lihong XuYong Zheng
Aug 29, 2013·World Journal of Gastroenterology : WJG·Catherine M GreeneMatthew W Lawless
Oct 11, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Stuart C GordonUNKNOWN CHeCS Investigators
Dec 11, 2013·PloS One·Prudence IveDebika Bhattacharya
Apr 9, 2014·BMC Infectious Diseases·Mauro AndreottiSandro Mancinelli
Jul 16, 2014·Gut·Giuliana AmaddeoJessica Zucman-Rossi
Dec 3, 2014·Journal of Ethnopharmacology·Ying JiangXue-Jiang Wang
Dec 30, 2014·Journal of Virology·Ningning LiuXin Ye
Dec 31, 2014·PLoS Medicine·Chunfeng QuZongtang Sun
May 4, 2015·Trends in Biochemical Sciences·Maria J MaciasJoan Massagué
Aug 22, 2015·Journal of Clinical Medicine·Sumadi Lukman Anwar, Ulrich Lehmann
Sep 18, 2015·World Journal of Gastroenterology : WJG·Ya-Kai Huang, Jian-Chun Yu
Sep 26, 2015·Biomedical Reports·Qian WuXing-Xing He
Sep 30, 2015·Oncotarget·Liangyou GuXu Zhang
Citations
Jun 21, 2019·The Journal of International Medical Research·Yingyuan LiZhongxing Wang
Oct 4, 2019·Scientific Reports·Rodrigo RiedelJulieta L Maymó
Feb 7, 2018·PloS One·Cynthia de la FuenteKylene Kehn-Hall
Mar 7, 2020·BMC Medical Genetics·Xiaojie LiYan Wang
Oct 22, 2020·Critical Reviews in Biochemistry and Molecular Biology·Charlotte de Ceuninck van CapellePeter Ten Dijke