The inhibition of PI3K and NFκB promoted curcumin-induced cell cycle arrest at G2/M via altering polyamine metabolism in Bcl-2 overexpressing MCF-7 breast cancer cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Özge BerrakNarçin Palavan-Ünsal

Abstract

Bcl-2 protein has been contributed with number of genes which are involved in oncogenesis. Among the many targets of Bcl-2, NFκB have potential role in induction of cell cycle arrest. Curcumin has potential therapeutic effects against breast cancer through multiple signaling pathways. In this study, we investigated the role of curcumin in induction of cell cycle arrest via regulating of NFκB and polyamine biosynthesis in wt and Bcl-2+ MCF-7 cells. To examine the effect of curcumin on cell cycle regulatory proteins, PI3K/Akt, NFκB pathways and polyamine catabolism, we performed immunoblotting assay. In addition, cell cycle analysis was performed by flow cytometry. The results indicated that curcumin induced cell cycle arrest at G2/M phase by downregulation of cyclin B1 and Cdc2 and inhibited colony formation in MCF-7wt cells. However, Bcl-2 overexpression prevented the inhibition of cell cycle associated proteins after curcumin treatment. The combination of LY294002, PI3K inhibitor, and curcumin induced cell cycle arrest by decreasing CDK4, CDK2 and cyclin E2 in Bcl-2+ MCF-7 cells. Moreover, LY294002 further inhibited the phosphorylation of Akt in Bcl-2+ MCF-7 cells. Curcumin could suppress the nuclear transport of NFκB through ...Continue Reading

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Citations

Dec 21, 2017·Medical Sciences : Open Access Journal·Tracy Murray-Stewart, Robert A Casero
Aug 30, 2018·Cellular and Molecular Neurobiology·Guiling ChenJun Dong
Feb 20, 2019·Journal of Biochemical and Molecular Toxicology·Yomna S Mo'menMohamed A Kandeil
Apr 15, 2017·BioMed Research International·Jian ChenWulan Li
Nov 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nehal GuptaSahdeo Prasad
Sep 1, 2021·European Journal of Pharmacology·Qi-Zhang LiYan Ji

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