PMID: 11900855Mar 20, 2002Paper

The inhibition of tryptophan hydroxylase, not protein synthesis, reduces the brain trapping of alpha-methyl-L-tryptophan: an autoradiographic study

Neurochemistry International
Yoshihiro TohyamaMirko Diksic

Abstract

The effects of the tryptophan hydroxylase (TPH) inhibitor p-chlorophenylalanine (PCPA; 200mg/kg; 3 days), and of the protein synthesis inhibitor cycloheximide (CXM, 2mg/kg), on regional serotonin (5-HT) synthesis were studied using the alpha-[14C]methyl-L-tryptophan (alpha-[14C]MTrp) autoradiographic method. The objectives of these investigations were to evaluate the changes, if any, on 5-HT synthesis, as measured with alpha-MTrp method, following the inhibition of TPH by PCPA, or the inhibition of proteins synthesis by CXM. The rats were used in the tracer experiment approximately 24h after the last dose of PCPA was administered, and in the CXM experiments, they were used 30 min following a single injection of CXM. In both experiments, the control rats were injected with the same volume of saline (0.5 ml/kg; s.c.) and at the same times as the drug injections. The results demonstrate that trapping of alpha-MTrp, which is taken to be related to brain 5-HT synthesis, is drastically reduced (40-80%) following PCPA treatment. The inhibition of protein synthesis with CXM did not have a significant effect on the global brain trapping of alpha-MTrp and 5-HT synthesis. These findings suggest that the brain trapping of alpha-[14C]MTrp r...Continue Reading

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Citations

Mar 9, 2011·Archives of General Psychiatry·Alexandre BerneyChawki Benkelfat
Mar 26, 2004·Psychoneuroendocrinology·Eva MikicsJózsef Haller
Oct 2, 2001·Journal of Neurochemistry·M Diksic, S N Young
Feb 19, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Csaba JuhászDiane C Chugani
Nov 30, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Anniek K D VisserRudi A J O Dierckx
Aug 5, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Csaba JuhászHarry T Chugani
Aug 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Pinelopi LundquistBengt Långström
Mar 8, 2005·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Sreenivasa R ChandanaDiane C Chugani
Mar 27, 2012·Nuclear Medicine and Biology·Csaba JuhászOtto Muzik

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