The inhibitory effect of 4-hydroxy-nonenal on DNA-polymerases alpha and beta from rat liver and rapidly dividing Yoshida ascites hepatoma

Cell Biochemistry and Function
E WawraE Schauenstein

Abstract

The purpose of this study was to determine firstly whether the isolated enzyme DNA polymerase alpha, which functions within the DNA replicase system, exhibits different sensitivity against the thiol-blocking agent 4-hydroxy-nonenal (HNE) when adult rat liver and the rapidly dividing Yoshida ascites hepatoma were used as enzyme sources and, secondly, whether the reaction catalysed by DNA polymerase is the most sensitive step of the DNA replicase system of native cells. DNA polymerase alpha as well as the non-replicative DNA polymerase beta, isolated from both sources, were remarkably similar with regard to their sensitivity against HNE, as indicated by the incorporation of radioactive label from [3H]deoxy-thymidine-triphosphate into DNA. The transport of [14C]thymidine through the plasma membrane and the incorporation of this precursor into DNA were studied with neonatal hepatocytes and with hepatoma cells. The incorporation of thymidine was inhibited at lower concentrations of HNE in both cell lines than the transport process and the reaction catalysed by DNA polymerase alpha. It was concluded that in the DNA replicase system of native liver and hepatoma cells another process different from the reaction catalysed by DNA polymer...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·E Wawra, I Dolejs
Mar 1, 1983·Molecular and Cellular Biology·E Wawra, E Wintersberger
Sep 1, 1982·European Journal of Biochemistry·R G WickremasingheA V Hoffbrand

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Citations

Jan 13, 2006·BioFactors·C CrifòC Salerno
Jan 1, 1987·Chemico-biological Interactions·M W Lame', H J Segall
Jan 1, 1991·Chemico-biological Interactions·A MigliettaL Gabriel
Jan 25, 1996·Chemistry and Physics of Lipids·A Mlakar, G Spiteller
Apr 1, 1988·Mechanisms of Ageing and Development·M PootJ F Jongkind
Jan 1, 1991·Free Radical Biology & Medicine·I EmeritH Esterbauer
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Jan 1, 1996·Free Radical Biology & Medicine·W G SiemsF J van Kuijk
Aug 18, 2000·Free Radical Biology & Medicine·E A Meagher, G A FitzGerald
Jan 1, 1989·Free Radical Research Communications·R H Burdon, C Rice-Evans
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·R Lindahl
Jul 1, 1994·Rapid Communications in Mass Spectrometry : RCM·B A BruennerJ B German
Mar 1, 1992·Cell Biochemistry and Function·A OliveroL Gabriel
Apr 1, 1990·Cell Biochemistry and Function·A OliveroL Gabriel
Jun 19, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A IndartB Bonet
Jul 26, 2011·Free Radical Biology & Medicine·Tanea T Reed
Mar 4, 1992·Molecular and Cellular Biochemistry·P Zahradka
Jan 1, 1992·Biological Trace Element Research·M SimonoffG Simonoff
Jan 1, 1992·Biological Trace Element Research·R A Anderson
Jan 1, 1992·Biological Trace Element Research·E G Offenbacher
Nov 1, 1992·Scandinavian Journal of Clinical and Laboratory Investigation·S KuboiK Kida
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·P A Bullock
Aug 1, 1995·Chemistry and Physics of Lipids·A Loidl-StahlhofenG Spiteller
Oct 1, 1993·Critical Reviews in Oncology/hematology·M U Dianzani

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