The inhibitory effect of celecoxib on mouse hepatoma H22 cell line on the arachidonic acid metabolic pathway

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Zhigang XuLi Chen

Abstract

Celecoxib is a selective inhibitor of cyclooxygenase-2 (COX-2). It may reduce the risk of cancer formation by affecting the metabolism of arachidonic acid (AA), which has been implicated in the development of cancer. Accordingly, this study was designed to determine the effects of celecoxib on the AA pathway in mouse hepatoma H22 cells. Celecoxib was found to inhibit the proliferation of H22 cells in a dose- and time-dependent manner. Low doses (50 and 100 micromol.L-1) of celecoxib caused an increase in the expression of cytosolic phospholipase A2 (cPLA2), but did not affect the expression of COX-2 in terms of the mRNA and protein levels. Surprisingly, the amount of AA was elevated and the level of prostaglandin E2 (PGE2) was unaltered in the culture supernatant. At higher celecoxib doses (200 and 400 micromol.L-1), the mRNA and protein of both COX-2 and cPLA2 were inhibited. The concentration of AA was increased, and PGE2 level was depressed in H22 cells. The ratio of AA to PGE2 was increased in a dose-dependent manner. Our findings suggest that the imbalance between AA and PGE2, characterized by increased AA at a low dosage and decreased PGE2 at a high dosage of celecoxib, was an important indicator of cytotoxicity of celeco...Continue Reading

References

Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H KogaM Sata
May 20, 1999·Annual Review of Pharmacology and Toxicology·J BalsindeE A Dennis
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·E Fosslien
May 22, 2001·Archives of Biochemistry and Biophysics·A Sultan, P M Sokolove
Jun 20, 2001·The Journal of Clinical Investigation·W L Smith, R Langenbach
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E NiederbergerG Geisslinger
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·D M ParkinP Pisani
Mar 2, 2002·Journal of Biochemistry·Makoto Murakami, Ichiro Kudo
Mar 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chang HanTong Wu
Mar 20, 2002·Nature Reviews. Cancer·R A Gupta, R N Dubois
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Jidong ChengToshikazu Hada
Sep 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael André KernPeter Schirmacher
Jun 11, 2003·Progress in Experimental Tumor Research·John A Baron
Aug 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jing LengTong Wu
Jun 14, 2005·Biochemical Pharmacology·Khosrow Kashfi, Basil Rigas
Nov 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wei CuiKe-Qin Hu
Dec 13, 2005·Cancer Treatment Reviews·Tong Wu
Jun 8, 2006·Journal of the National Cancer Institute·Sabine GröschGerd Geisslinger
Oct 21, 2006·Biochimica Et Biophysica Acta·Masako Nakanishi, Daniel W Rosenberg
Feb 6, 2007·Journal of Hepatology·Jaideep BehariSatdarshan P S Monga

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Citations

Oct 11, 2012·World Journal of Gastroenterology : WJG·Hsu-Heng YenChin-San Liu
Aug 15, 2013·Journal of Lipid Research·Robert RamerBurkhard Hinz
Feb 12, 2014·Applied Optics·Jinan XiaAndrea M Rossi
Aug 6, 2011·Biosensors & Bioelectronics·A M GiovannozziA M Rossi

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