The inhibitory effect of cyclophosphamide on camel retina acetylcholinesterase activity

Toxicology Letters
F al-KhwyterA A Al-Jafari

Abstract

Kinetic parameters for the effect of cyclophosphamide (CP) on the camel retina acetylcholinesterase (AChE) activity were investigated for the first time in the present study. It was found that 18 micrograms of retina protein and an incubation time of 4.0 min were suitable conditions for linear of AChE activity. The CP effect was independent of time of incubation with AChE before the addition of substrate, which shows it reversible action. Moreover, dilution data prove that CP is a reversible inhibitor of camel retina AChE. Cyclophosphamide (0.2-2.4 mM) inhibited activity of camel retina in a concentration-dependent fashion, the IC50 being about 1.17 mM. The Michaelis constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.106 mM and the Vmax was 0.765 mumol/min/mg protein. Dixon as well as Lineweaver-Burk plots and their secondary replots indicated that the nature of the inhibition was of the pure noncompetitive type. The value of Ki was estimated as 0.763 mM by the primary Dixon and secondary replots of the Lineweaver-Burk plot.

References

Jan 16, 1999·Journal of Biochemical and Molecular Toxicology·M A Kamal, A A Al-Jafari
Mar 16, 2013·Applied Biochemistry and Biotechnology·Habibe Budak KayaHasan Ozdemir
Mar 21, 1998·Journal of Enzyme Inhibition·A S AlhomidaA A Al-Jafari
Feb 1, 1997·Journal of Enzyme Inhibition·A A Al-JafariA S Alhomida

Citations

Apr 1, 1976·Canadian Journal of Biochemistry·G TunnicliffA Barbeau
Oct 1, 1979·The Journal of Cell Biology·R H Masland, J W Mills
Jul 16, 1990·Brain Research·L S JenW Z Shen
Aug 1, 1990·The Journal of Laryngology and Otology·M Z SiodlakM Squadrelli-Saraceno
Oct 2, 1990·Neuroscience Letters·R M Dawson
Sep 1, 1995·The International Journal of Biochemistry & Cell Biology·A A Aljafari
Feb 1, 1995·Experimental Eye Research·J B HutchinsV E Jefferson
Jan 19, 1995·Toxicology·A A Al-JafariM A Kamal
Jun 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·A A Al-Jafari
Jan 1, 1994·Cell Biochemistry and Function·A A Al-Jafari, M A Kamal
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

Related Concepts

Cyclophosphamide
Entire Retina
Log-Linear Models
ACHE
Antineoplastic Agents, Alkylating
Retinal Diseases
Cholinesterase Inhibitors, Reversible
Acetylthiocholine iodide
Malignant Neoplasm of Retina
Retina

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