PMID: 19149672Jan 20, 2009Paper

The inhibitory effect of propofol on bovine lactoperoxidase

Protein and Peptide Letters
M SişecioğluHasan Ozdemir

Abstract

Propofol (2,6-diisopropylphenol) is a hypnotic intravenous agent with in vivo antioxidant properties. This study was undertaken to examine the in vitro effect of propofol on lactoperoxidase (LPO; E.C. 1.11.1.7) obtained from bovine milk. Lactoperoxidase was purified with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. Lactoperoxidase was purified with a yield of 21.6%, a specific activity of 34 EU/mg proteins and 14.7-fold purification. One enzyme unit is defined as the oxidation of 1 micromol ABTS per min under the assay condition (25( degrees )C, pH: 6.0). To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The effect of propofol on lactoperoxidase were determined using 2,2'-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate. The IC(50) value of propofol was found as 15.97 microM. Also, K(i) constant for propofol was 3.72 microM and propofol was found as competitive inhibitor.

Citations

Feb 4, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Melda ŞişecioğluHasan Özdemir
Sep 10, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·S Beyza Ozturk SarikayaHasan Ozdemir
May 29, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Hulya GocerClaudiu T Supuran
Jul 3, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Parham TaslimiClaudiu T Supuran
Jul 7, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Fevzi TopalSaleh H Alwasel
Apr 22, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Hulya GocerClaudiu T Supuran
Jun 26, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Hülya GöçerClaudiu T Supuran
Dec 17, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Zübeyir Huyutİlhami Gülçin
Nov 21, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Afsun SujayevClaudiu T Supuran
Jul 25, 2015·Journal of Enzyme Inhibition and Medicinal Chemistry·Leyla Polat KoseClaudiu T Supuran
Dec 30, 2015·International Journal of Biological Macromolecules·Cevahir AltinkaynakNalan Özdemir
Mar 18, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Süleyman YılmazClaudiu T Supuran
Nov 6, 2012·Food Chemistry·Ali AtaseverO Irfan Kufrevioglu
Apr 13, 2015·Bioorganic & Medicinal Chemistry·Alper YıldırımClaudiu T Supuran
Sep 24, 2013·Environmental Toxicology and Pharmacology·Levent MercanDeniz Ekinci
Oct 25, 2016·International Journal of Molecular Sciences·Hayriye GençHasan Özdemir
Nov 18, 2016·Applied Biochemistry and Biotechnology·Esmail M El-FakharanyElrashdy M Redwan
Jan 12, 2011·Journal of Food Protection·Ahmad Nimatullah Al-BaarriShigeru Hayakawa
Jun 9, 2017·Journal of Biochemical and Molecular Toxicology·Zeynep KöksalHasan Özdemir
May 26, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zeynep KöksalHasan Ozdemir
Aug 22, 2018·Drug and Chemical Toxicology·Zeynep Köksal, Zuhal Alim
May 22, 2010·Chemical Biology & Drug Design·S Beyza Oztürk SarikayaClaudiu T Supuran
Dec 27, 2019·Journal of Food Biochemistry·Zeynep KoksalHasan Ozdemir
Jan 7, 2020·Mini Reviews in Medicinal Chemistry·Hulya Akıncıoğlu, İlhami Gülçin
Aug 29, 2020·Journal of Biomolecular Structure & Dynamics·Ramazan Kalinİlhami Gülçin
Mar 25, 2019·International Journal of Molecular Sciences·Marcin MagaczWirginia Krzyściak
Nov 16, 2019·Journal of Biochemical and Molecular Toxicology·Ramazan KalinHasan Ozdemir

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