The inhibitory effect of quercitrin gallate on iNOS expression induced by lipopolysaccharide in Balb/c mice.

Journal of Veterinary Science
Hyun Ye JoByeongwoo Ahn

Abstract

Quercetin 3-O-beta-(2(")-galloyl)-rhamnopyranoside (QGR) is a naturally occurring quercitrin gallate, which is a polyphenolic compound that was originally isolated from Persicaria lapathifolia (Polygonaceae). QGR has been shown to have an inhibitory effect on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Therefore, this study was conducted to investigate the inhibitory effect of QGR on nitric oxide production and inducible nitric oxide synthases (iNOS) expression in LPS-stimulated Balb/c mice. To accomplish this, 10 mg/kg of QGR was administered via gavage once a day for 3 days. iNOS was then induced by intraperitoneal injection of LPS. Six hours after the LPS treatment the animals were sacrificed under ether anethesia. The serum levels of NO were then measured to determine if QGR exerted an inhibitory effect on NO production in vivo. LPS induced an approximately 6 fold increase in the expression of NO. However, oral administration of QGR reduced the LPS induced increase in NO by half. Furthermore, RT-PCR and western blot analysis revealed that the increased levels of iNOS expression that occurred in response to treatment with LPS were significantly attenuated in response to QGR...Continue Reading

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Citations

Mar 25, 2011·Critical Reviews in Food Science and Nutrition·R GonzálezF Sánchez de Medina
Apr 23, 2011·Bioscience, Biotechnology, and Biochemistry·Kayoko KawakamiMasao Hirayama
Aug 10, 2012·Canadian Journal of Physiology and Pharmacology·Upa KukongviriyapanWanida Donpunha
Feb 21, 2016·The Journal of Nutritional Biochemistry·Asmahan ImessaoudeneMourad Elhabiri
Mar 18, 2011·Phytochemical Analysis : PCA·Kayoko KawakamiMasao Hirayama
Dec 19, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·You-Chang OhJin Yeul Ma
Dec 31, 2014·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Mostafa M El-ShafeyAmr D Mariee
Dec 18, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·You-Chang OhJin Yeul Ma
Jun 30, 2012·The American Journal of Chinese Medicine·You-Chang OhJin Yeul Ma
Jul 20, 2012·The American Journal of Chinese Medicine·You-Chang OhJin Yeul Ma
Dec 23, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Irina C ChisSimona Clichici
Oct 19, 2020·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tae Woo OhKyungho Kim

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Methods Mentioned

BETA
nuclear translocation
ELISA
PCR
electrophoresis

Software Mentioned

Quantity One
SPSS

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