The Inhibitory Effect of Validamycin A on Aspergillus flavus

International Journal of Microbiology
Napasawan PlabutongArsa Thammahong

Abstract

Aspergillus flavus is one of the most common isolates from patients with fungal infections. Aspergillus infection is usually treated with antifungal agents, but side effects of these agents are common. Trehalase is an essential enzyme involved in fungal metabolism, and the trehalase inhibitor, validamycin A, has been used to prevent fungal infections in agricultural products. In this study, we observed that validamycin A significantly increased trehalose levels in A. flavus conidia and delayed germination, including decreased fungal adherence. In addition, validamycin A and amphotericin B showed a combinatorial effect on A. flavus ATCC204304 and clinical isolates with high minimum inhibitory concentrations (MICs) of amphotericin B using checkerboard assays. We observed that validamycin A and amphotericin B had a synergistic effect on A. flavus strains resistant to amphotericin B. The MICs in the combination of validamycin A and amphotericin B were at 0.125 μg/mL and 2 μg/mL, respectively. The FICI of validamycin A and amphotericin B of these clinical isolates was about 0.25-0.28 with synergistic effects. No drug cytotoxicity was observed in human bronchial epithelial cells treated with validamycin A using LDH-cytotoxicity assay...Continue Reading

References

Apr 1, 1987·The Journal of Antibiotics·N AsanoK Matsui
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·A D Elbein
Dec 22, 1995·Journal of Medicinal Chemistry·M YamaguchiM Hirama
Jul 1, 1996·Antimicrobial Agents and Chemotherapy·J R Perfect
Oct 17, 1998·Journal of Clinical Microbiology·J A van BurikD H Spach
Feb 13, 2001·Plant Physiology·J MüllerA Wiemken
Mar 15, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·T MahmudH G Floss
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Avid BrodmannAstrid Wingler
Mar 11, 2003·Glycobiology·Alan D ElbeinDavid Carroll
Aug 13, 2003·Eukaryotic Cell·George J G RuijterJaap Visser
Nov 12, 2003·The Journal of Experimental Biology·Hans Merzendorfer, Lars Zimoch
May 7, 2004·Applied and Environmental Microbiology·Matthieu JulesJean-Luc Parrou
Jul 15, 2004·The Journal of Biological Chemistry·Yolanda PedreñoEulogio Valentin
May 19, 2007·Medical Mycology·Joshua PerlrothBrad Spellberg
May 29, 2007·Microbiology·M T HedayatiD W Denning
Feb 9, 2008·Annual Review of Plant Biology·Matthew J PaulYuhua Zhang
May 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ben De PauwUNKNOWN National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group
Aug 12, 2008·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Arunaloke ChakrabartiM R Shivaprakash
Jan 21, 2009·Drug Discovery Today·Katharina GauwerkyHans C Korting
Feb 12, 2009·Mycoses·Suganthini KrishnanPranatharthi H Chandrasekar
Oct 20, 2009·Revista iberoamericana de micología·Rafael Laniado-Laborín, Maria Noemí Cabrales-Vargas
Jan 9, 2010·Medical Mycology·Wendy W J van de SandeIrma A J M Bakker-Woudenberg
May 5, 2010·Infection and Immunity·Nadia Al-BaderDonald C Sheppard
Aug 20, 2010·Medical Mycology·Arunaloke ChakrabartiM R Shivaprakash
Nov 6, 2012·The New Phytologist·Aarón BarrazaFederico Sanchez
Dec 12, 2012·Microbes and Infection·Kelly M ShepardsonRobert A Cramer
Mar 2, 2013·Studies in Mycology·M R van LeeuwenJ Dijksterhuis

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Citations

Nov 5, 2020·Memórias do Instituto Oswaldo Cruz·Rafael G LopesBoris U Stambuk
Apr 1, 2021·Pest Management Science·María-Dolores García, Juan-Carlos Argüelles

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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