The Inhibitory Effects of Djulis (Chenopodium formosanum ) and Its Bioactive Compounds on Adipogenesis in 3T3-L1 Adipocytes

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Charng-Cherng ChyauPin-Der Duh

Abstract

The aim of this study was to provide new insights into the role of the ethanolic extracts of Djulis (Chenopodium formosanum, EECF) and its bioactive compounds in preventing adipogenesis in 3T3-L1 adipocytes. The results demonstrated EECF significantly inhibited oil red O-stained material (OROSM), triglyceride levels and glycerol-3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 adipocytes. The expression of the critical molecules involved in lipid synthesis such as PPARγ, C/EBPα and SREBP-1c was attenuated in EECF-treated cells. According to HPLC-DAD and HPLC-MS/MS analysis, rutin, kaempferol, betanin and another nine compounds were present in EECF. The suppression of lipid accumulation by rutin, kaempferol and betanin occurred by decreasing the gene expression of PPARγ, C/EBPα and SREBP-1c. Taken together, these findings suggest the presence of bioactive compounds in EECF may partly account for the anti-adipogenesis of EECF and EECF is therefore a potentially lipid lowering functional food.

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Citations

Apr 26, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·John F Lechner, Gary D Stoner
Oct 16, 2020·Critical Reviews in Food Science and Nutrition·Arakkaveettil Kabeer FarhaHarold Corke

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Methods Mentioned

BETA
PCR
protein assay
Assay
Electrophoresis

Software Mentioned

Image J
SPSS

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