The inhibitory effects of Geranium thunbergii on interferon-γ- and LPS-induced inflammatory responses are mediated by Nrf2 activation

International Journal of Molecular Medicine
Hee-Jin ChoiKi-Tae Ha

Abstract

Geranium thunbergii Sieb. et Zucc. (GT; which belongs to the Geraniaceae family) has been used as a traditional medicine in East Asia for the treatment of inflammatory diseases, including arthritis and diarrhea. However, the underlying mechanisms of the anti-inflammatory effects of GT remain poorly understood. In the present study, we examined the mechanisms responsible for the anti-inflammatory activity of GT in macrophages. The results revealed that GT significantly inhibited the lipopolysaccharide (LPS)- and interferon-γ (IFN-γ)-induced expression of pro-inflammatory genes, such as inducible nitric oxide synthase, tumor necrosis factor-α and interleukin-1β, as shown by RT-PCR. However, the inhibitory effects of GT on LPS- and IFN-γ-induced inflammation were associated with an enhanced nuclear factor erythroid 2-related factor 2 (Nrf2) activity, but not with the suppression of nuclear factor (NF)-κB activity, as shown by western blot analysis. In addition, in bone marrow-derived macrophages (BMDM) isolated from Nrf2 knockout mice, GT did not exert any inhibitory effect on the LPS- and IFN-γ-induced inflammation. Taken together, our findings indicate that the anti-inflammatory effects of GT may be associated with the activatio...Continue Reading

References

Sep 3, 1999·The Journal of Biological Chemistry·J AlamJ L Cook
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·K Chan, Y W Kan
Nov 18, 2003·The Journal of Pharmacology and Experimental Therapeutics·Nieves MárquezEduardo Muñoz
Oct 13, 2004·Biochemical Pharmacology·Andrea J MoreiraJavier González-Gallego
Jan 5, 2005·BioFactors·Wang XiufenMai Matsubara
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Riku KorhonenEeva Moilanen
Aug 22, 2006·Immunobiology·Kate SchroderDavid A Hume
Jan 18, 2007·Archives of Pharmacal Research·Qing-He LiuEun-Rhan Woo
Aug 22, 2007·Complementary Therapies in Medicine·Mitchell KaplanAli Keshavarzian
Oct 25, 2008·Arthritis Research & Therapy·Stefan K DrexlerBrian M Foxwell
Dec 17, 2008·Annals of the New York Academy of Sciences·Jeffrey A JohnsonPei-Chun Chen
Jun 17, 2009·Current Opinion in Gastroenterology·Martina E Spehlmann, Lars Eckmann
Mar 23, 2010·Cell·Carl Nathan, Aihao Ding
Apr 7, 2010·Antioxidants & Redox Signaling·Nobunao WakabayashiThomas W Kensler
May 25, 2010·Biochimica Et Biophysica Acta·Subash C GuptaBharat B Aggarwal
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal
Oct 5, 2010·Annual Review of Pharmacology and Toxicology·Debra L LaskinJeffrey D Laskin
Mar 3, 2011·Archives of Toxicology·Liam Baird, Albena T Dinkova-Kostova
Mar 17, 2011·Antioxidants & Redox Signaling·Nuria MaicasMaria José Alcaraz
Apr 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Jun-Young ChoiMyungsoo Joo
May 4, 2012·Archives of Pharmacal Research·Jin-Ah LeeHyeun Kyoo Shin
Sep 22, 2012·Trends in Cell Biology·Laura TornatoreGuido Franzoso
Dec 4, 2012·Rheumatology·Jean-Luc DavignonAlain Cantagrel
Feb 4, 2014·Biochemical and Biophysical Research Communications·Bin LiTaixing Cui

❮ Previous
Next ❯

Citations

Sep 1, 2017·Food & Function·Marcelo D CatarinoSusana M Cardoso
Aug 30, 2019·Current Pharmaceutical Design·Amirhossein DavoodvandiHamed Mirzaei
May 1, 2018·Oncotarget·Uzma SaqibRajkumar Savai
Jan 17, 2020·Current Pharmaceutical Design·Vânia C GraçaPaulo F Santos
Jan 21, 2016·Fundamental & Clinical Pharmacology·Boxin ShangLei Dong
Jul 16, 2015·Clinical and Experimental Pharmacology & Physiology·Feng ZhaoMin Zhao
May 8, 2020·International Journal of Molecular Sciences·Ji Hye KimHwan-Suck Chung
Feb 26, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Prakash Raj PandeyaHyun-Ju Jung
Jul 3, 2021·Journal of Ethnopharmacology·Myong Hak RiXuejun Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein assay
electrophoresis
nuclear translocation

Software Mentioned

ImageJ
LC solution

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here