The inhibitory effects of PGG and EGCG against the SARS-CoV-2 3C-like protease.

Biochemical and Biophysical Research Communications
Wei-Chung ChiouCheng Huang


The coronavirus disease (COVID-19) pandemic, resulting from human-to-human transmission of a novel severe acute respiratory syndrome coronavirus (SARS-CoV-2), has led to a global health crisis. Given that the 3 chymotrypsin-like protease (3CLpro) of SARS-CoV-2 plays an indispensable role in viral polyprotein processing, its successful inhibition halts viral replication and thus constrains virus spread. Therefore, developing an effective SARS-CoV-2 3CLpro inhibitor to treat COVID-19 is imperative. A fluorescence resonance energy transfer (FRET)-based method was used to assess the proteolytic activity of SARS-CoV-2 3CLpro using intramolecularly quenched fluorogenic peptide substrates corresponding to the cleavage sequence of SARS-CoV-2 3CLpro. Molecular modeling with GEMDOCK was used to simulate the molecular interactions between drugs and the binding pocket of SARS-CoV-2 3CLpro. This study revealed that the Vmax of SARS-CoV-2 3CLpro was about 2-fold higher than that of SARS-CoV 3CLpro. Interestingly, the proteolytic activity of SARS-CoV-2 3CLpro is slightly more efficient than that of SARS-CoV 3CLpro. Meanwhile, natural compounds PGG and EGCG showed remarkable inhibitory activity against SARS-CoV-2 3CLpro than against SARS-CoV 3...Continue Reading


Mar 30, 2004·Proteins·Jinn-Moon Yang, Chun-Chen Chen
Aug 11, 2011·Biochemical Pharmacology·Brahma N SinghRakesh K Srivastava
Feb 16, 2016·FEBS Letters·Peter KumbergerFrederik Graw
Jun 28, 2016·Nature Reviews. Microbiology·Emmie de WitVincent J Munster
Aug 10, 2017·BioMed Research International·Andreia GranjaSalette Reis
Nov 11, 2017·Nucleic Acids Research·David S WishartMichael Wilson
Nov 15, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Seri JoMi-Sun Kim
Feb 20, 2020·The New England Journal of Medicine·Lirong ZouJie Wu
Feb 29, 2020·The New England Journal of Medicine·Wei-Jie GuanUNKNOWN China Medical Treatment Expert Group for Covid-19
Mar 5, 2020·Nature Reviews. Drug Discovery·Guangdi Li, Erik De Clercq
Mar 17, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xin Liu, Xiu-Jie Wang
Mar 21, 2020·Lancet·Puja MehtaUNKNOWN HLH Across Speciality Collaboration, UK
Sep 24, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Minsu JangJunsoo Park

❮ Previous
Next ❯


Apr 9, 2021·The Journal of General Virology·Lisa HenssBarbara S Schnierle
Aug 31, 2021·Computational and Structural Biotechnology Journal·Fangfang Yan, Feng Gao
Sep 14, 2021·Frontiers in Pharmacology·Wei-Chung ChiouCheng Huang

❮ Previous
Next ❯

Methods Mentioned

fluorescence resonance

Software Mentioned

Spark® Control [UNK]
GraphPad Prism

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.