PMID: 9654098Jul 8, 1998Paper

The initial kinetics of hydrolysis by cellobiohydrolases I and II is consistent with a cellulose surface-erosion model

European Journal of Biochemistry
Priit VäljamäeG Johansson

Abstract

Introduction of a novel method for the quantification of the cellobiose released made it possible to follow the initial stage of hydrolysis of bacterial microcrystalline cellulose (BMCC) by cellobiohydrolases 1,4-beta-D-glucan-cellobiohydrolase I (CBH I) and 1,4-beta-D-glucan-cellobiohydrolase II (CBH II) from Trichoderma reesei. A drastic retardation of the rate of the hydrolysis was observed already at a very low degree of conversion. Earlier-suggested retardation factors, such as product inhibition by cellobiose or enzyme inactivation, could be discounted as primary causes for the pattern. A model including steric hindrance by non-productive binding and erosion of the cellulose surface during the processive action of exoenzymes was proposed to describe the rate retardation observed. Simultaneous action of CBH I and CBH II on cellulose was not a prerequisite for synergy between them.

Citations

Aug 31, 2010·Applied Biochemistry and Biotechnology·Søren N OlsenPeter Westh
Oct 13, 2011·Applied Biochemistry and Biotechnology·Li-Huan QiuJian-He Xu
Aug 17, 1999·Current Opinion in Biotechnology·M E HimmelC E Wyman
Aug 15, 2001·European Journal of Biochemistry·P VäljamäeG Johansson
Apr 12, 2012·The Journal of Biological Chemistry·Nicolaj Cruys-BaggerPeter Westh
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Sep 15, 2012·Enzyme and Microbial Technology·Marcos Henrique Luciano SilveiraJürgen Andreaus
Dec 28, 2011·Bioresource Technology·Zhuoliang YeR Eric Berson
Oct 18, 2011·Bioresource Technology·Zhuoliang Ye, R Eric Berson
Jul 7, 2009·Biotechnology Advances·Prabuddha BansalAndreas S Bommarius
Jun 22, 2013·The FEBS Journal·Nicolaj Cruys-BaggerPeter Westh
Jul 24, 2008·Metabolic Engineering·Andreas S BommariusYunqiao Pu
Jun 16, 2009·Biotechnology and Bioengineering·Wen ZhouHeinz-Bernd Schüttler
Dec 9, 2008·Biotechnology and Bioengineering·Rajesh Gupta, Y Y Lee
May 1, 2007·Biotechnology Progress·Suma PeriM Nazmul Karim
May 30, 2006·Biotechnology and Bioengineering·Bin YangCharles E Wyman
Mar 5, 2011·The FEBS Journal·Eigil PraestgaardPeter Westh
May 28, 2010·Biotechnology and Bioengineering·Jürgen Jalak, Priit Väljamäe
Mar 5, 2013·Biotechnology and Bioengineering·Patricia BubnerBernd Nidetzky
May 28, 2010·Biotechnology and Bioengineering·Seth E LevineDouglas S Clark
Jul 7, 2012·Biotechnology and Bioengineering·Nicolaj Cruys-BaggerPeter Westh
Feb 13, 2010·The FEBS Journal·Mélanie HallAndreas S Bommarius
May 13, 2006·Biotechnology Advances·Y-H Percival ZhangJonathan R Mielenz
Feb 16, 2013·Enzyme and Microbial Technology·Leigh MurphyPeter Westh
Jan 15, 2015·Parasites & Vectors·Sahreena LakhundiNaveed Ahmed Khan
Apr 8, 2015·Bioresource Technology·Bianca Consorti BussamraAline Carvalho da Costa
Jun 5, 2012·Carbohydrate Polymers·Longyun HaoRongzhan Liu
Sep 11, 2003·Biotechnology and Bioengineering·Priit VäljamäeGunnar Johansson
Dec 6, 2018·Biotechnology and Bioengineering·Zdeneˇk PetrášekBernd Nidetzky
May 11, 2006·Biotechnology and Bioengineering·Y-H Percival Zhang, Lee R Lynd

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