The initiation of calcium release following muscarinic stimulation in rat lacrimal glands.

The Journal of Physiology
A Marty, Y P Tan

Abstract

1. Acinar cells were isolated from rat lacrimal glands, and the Ca2+ release response of these cells was studied using two experimental approaches. In one approach, changes in Ca2+ concentration, Cai2+, were monitored by measuring Ca2(+)-dependent Cl- currents using tight-seal whole-cell recording. Alternatively, such changes were measured as a fluorescence signal in cells loaded with Fura-2. 2. Following bath application of ACh (0.5 microM), the cell current recorded at -60 mV was unchanged for ca 0.8 s, then rose in a biphasic manner. The initial phase of the current rise ('hump') took different appearances depending on the cell studied, and it sometimes stood out from the main part of the response as a partially isolated transient. 3. In cells which had been loaded with Fura-2, Cai2+ was found to rise abruptly following a silent period. The delay was larger if ACh (0.2-0.5 microM) was applied in a depolarizing isotonic K+ saline than if it was applied in the normal saline. In addition, the maximum of the Cai2+ response was reduced with depolarizing stimulating solutions. This indicates that membrane potential modulates the Cai2+ response. 4. Responses to 5 microM-ACh, a saturating agonist concentration, were almost identical...Continue Reading

Citations

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