The initiation of hepatocyte-specific gene expression within embryonic hepatocytes is a stochastic event.

Developmental Biology
M A van RoonW H Lamers

Abstract

To gain insight into the mechanisms that govern the first steps of liver-specific enzyme accumulation upon hormone exposure, the initial accumulation of carbamoylphosphate synthetase, phosphoenolpyruvate carboxykinase, and arginase in monolayer cultures of Embryonic Day 14 rat hepatocytes was studied. By using different fluorescent labels the initial accumulation of two enzymes could be studied simultaneously in individual cells. Both microscopic and flow cytometric analyses showed that the initial expression of genes that are under the same hormonal control appears to lack the coordinated regulation of expression that is seen later in development. The coordination is gradually established during exposure to hormones. Once gene expression becomes coordinated, the enzyme content appears to increase continuously with time. Therefore, we postulate that within individual embryonic hepatocytes the initial intercellular heterogeneity in rate of accumulation of a particular protein may be the result of competition of different genes for an initially limiting supply of common regulatory factors, leading to random differences in the rate of accumulation of the respective gene products. This makes the initiation of liver-specific gene ex...Continue Reading

References

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Citations

Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S FieringD I Martin
May 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M S Ko
Sep 23, 1991·Biochimica Et Biophysica Acta·C Van den BogertK M Sinjorgo
May 11, 2005·Nature Reviews. Genetics·Mads KaernJames J Collins
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M C WaltersD I Martin
Sep 10, 1998·Genes & Development·S NewlandsE C Hardeman
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·D L CookS J Tapscott
Nov 18, 2009·Progress in Biophysics and Molecular Biology·Jean-Jacques Kupiec
May 1, 1994·Differentiation; Research in Biological Diversity·M A DingemanseW H Lamers
Aug 1, 1989·Differentiation; Research in Biological Diversity·M A van RoonW H Lamers
Apr 28, 2009·Stem Cell Research·Julianne D HalleyDavid A Winkler
Dec 24, 2014·Cell and Tissue Research·Robert Durruthy-Durruthy, Stefan Heller
Dec 31, 1997·Microscopy Research and Technique·R G NotenboomW H Lamers
Apr 11, 2003·Nature·William J BlakeJ J Collins
Dec 17, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Quan Liu, Ya Jia
Oct 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W H LamersA F Moorman
Apr 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S H SigalP A Holst

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