Abstract
To study the thyroid regeneration and injury of recurrent laryngeal nerve after irreversible electroporation (IRE). 12 pigs were divided into three groups: six pigs underwent IRE, other pigs were used as controls. IRE was performed near tracheoesophageal groove, to ablate most part of thyroid gland. Parathyroid and thyroid function, recurrent laryngeal nerve injury and thyroid computed tomography (CT) imaging were regularly investigated. The histopathology results were analyzed to detect thyroid regeneration. Masson's trichrome method for collagen and immunohistochemistry were performed for Soluble protein-100 (S100) and neurofilaments on nerve section. In IRE group, there were no symptoms of recurrent laryngeal nerve-related injury. No abnormalities of recurrent laryngeal nerve were shown on hematoxylin-eosin (HE) staining, Masson's trichrome staining, Neurofilament (NF) staining and S100 staining. There were no significant changes for thyroid and parathyroid function in all pigs. Immediately after IRE, CT showed hypoattenuation in the ablated thyroid gland and it became swelling. 14 days after IRE, thyroid CT showed hetergenous attenuation in the electroporation zone, and the size and attenuation of thyroid gland were normal ...Continue Reading
References
Aug 3, 2007·Technology in Cancer Research & Treatment·Gary OnikBoris Rubinsky
Jan 12, 2010·International Journal of Surgery·Tamer YoussefEbraheim El-Dosoky
Oct 12, 2010·Thyroid : Official Journal of the American Thyroid Association·Roberto ValcaviClaudio M Pacella
Jun 7, 2011·Radiology·Helmut SchoellnastStephen B Solomon
Sep 17, 2011·AJNR. American Journal of Neuroradiology·J H ShinJ-H Kim
Sep 27, 2012·European Radiology·Helmut SchoellnastStephen B Solomon
Sep 28, 2012·Thyroid : Official Journal of the American Thyroid Association·Eun Ju HaYoung Kee Shong
Aug 21, 2013·The Journal of Clinical Endocrinology and Metabolism·Hossein GharibEnrico Papini
Oct 15, 2013·The Laryngoscope·Colin W FullerM Boyd Gillespie
Feb 25, 2014·Endocrine·Alessandro PuzzielloMaurizio De Palma
Dec 3, 2014·Journal of Vascular and Interventional Radiology : JVIR·Min Ji HongSuck Joon Hong
Jan 29, 2016·PeerJ·Alexander GolbergBasak E Uygun
Jun 9, 2016·Radiology·Alda L TamDavid T Fuentes
Aug 9, 2016·Journal of Translational Medicine·Sheng LiPeihong Wu
Dec 16, 2016·European Radiology·Cherry KimJung Hwan Baek
May 22, 2017·Langenbeck's Archives of Surgery·Alessandra SabaAdolfo Pisanu
May 11, 2019·BMC Surgery·Paolo Del RioFederico Cozzani