The inner CSF-brain barrier: developmentally controlled access to the brain via intercellular junctions

Frontiers in Neuroscience
Sophie WhishN R Saunders

Abstract

In the adult the interface between the cerebrospinal fluid and the brain is lined by the ependymal cells, which are joined by gap junctions. These intercellular connections do not provide a diffusional restrain between the two compartments. However, during development this interface, initially consisting of neuroepithelial cells and later radial glial cells, is characterized by "strap" junctions, which limit the exchange of different sized molecules between cerebrospinal fluid and the brain parenchyma. Here we provide a systematic study of permeability properties of this inner cerebrospinal fluid-brain barrier during mouse development from embryonic day, E17 until adult. Results show that at fetal stages exchange across this barrier is restricted to the smallest molecules (286Da) and the diffusional restraint is progressively removed as the brain develops. By postnatal day P20, molecules the size of plasma proteins (70 kDa) diffuse freely. Transcriptomic analysis of junctional proteins present in the cerebrospinal fluid-brain interface showed expression of adherens junctional proteins, actins, cadherins and catenins changing in a development manner consistent with the observed changes in the permeability studies. Gap junction p...Continue Reading

References

Jul 1, 1979·The Journal of Physiology·K M DziegielewskaN R Saunders
Jul 1, 1978·Journal of Neurochemistry·Q L Choo, D Bray
May 1, 1977·Developmental Biology·M E Desmond, A G Jacobson
Feb 1, 1992·The Journal of Cell Biology·M Ozawa, R Kemler
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K M DziegielewskaN R Saunders
Aug 1, 1988·Developmental Biology·T ThomasM Dziadek
Jan 1, 1988·Annals of the New York Academy of Sciences·H F Cserr
Jul 1, 1986·Neuropathology and Applied Neurobiology·K Møllgård, N R Saunders
Jun 1, 1987·Journal of Cellular Biochemistry·C A OteyJ C Bulinski
Mar 29, 1985·Biochemical and Biophysical Research Communications·P W DicksonG Schreiber
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K M DziegielewskaC H Tyndale-Biscoe
Feb 1, 1985·Brain Research·G FossanN R Saunders
Aug 1, 1985·Developmental Medicine and Child Neurology·M Adinolfi
Mar 1, 1969·The Journal of Cell Biology·M W Brightman, T S Reese
Mar 1, 1980·The Journal of Physiology·K M DziegielewskaN R Saunders
Apr 15, 1981·Developmental Biology·K M DziegielewskaN R Saunders
Mar 5, 1999·Clinical and Experimental Pharmacology & Physiology·N R SaundersK M Dziegielewska
Feb 26, 2000·Cellular and Molecular Neurobiology·N R SaundersK M Dziegielewska
Jan 30, 2002·Trends in Neurosciences·Katarzyna M Dziegielewska, Norman R Saunders
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Jan 1, 1962·Zeitschrift für Zellforschung und mikroskopische Anatomie·V M TENNYSON, G D PAPPAS
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Magdalena Götz, Wieland B Huttner
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson
Sep 29, 2006·The Journal of Biological Chemistry·William I Weis, W James Nelson
Jan 16, 2007·Cell·Ramsés AyalaLi-Huei Tsai
Feb 23, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Pia A JohanssonNorman R Saunders
Nov 27, 2008·Nucleic Acids Research·T J P HubbardP Flicek
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jingjing ZhangSalim Abdelilah-Seyfried
Sep 25, 2010·Journal of Neural Transmission·H C BauerH Bauer
Oct 4, 2011·Brain : a Journal of Neurology·Helen B StolpZoltán Molnár
Dec 27, 2011·Neurotoxicology·C Joakim EkNorman R Saunders
Jan 11, 2012·Progress in Brain Research·Zoltán Molnár, Gavin Clowry

❮ Previous
Next ❯

Citations

Apr 9, 2015·Frontiers in Neuroscience·Christian B BrøchnerKjeld Møllgård
Feb 12, 2017·Acta Neuropathologica·Yong GuoClaudia F Lucchinetti
Sep 14, 2017·Journal of Neuro-oncology·Ian David ConnollyMelanie Hayden Gephart
May 9, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Richard F KeepGuohua Xi
Feb 21, 2018·Frontiers in Neural Circuits·Andreas BjorefeldtEric Hanse
May 19, 2018·The Journal of Physiology·Norman R SaundersMark D Habgood
Feb 15, 2019·Journal of Leukocyte Biology·Vitka GresPhilipp Henneke
Aug 7, 2017·Cellular and Molecular Life Sciences : CMLS·Vladimir Korzh
Jul 3, 2020·Chemical & Pharmaceutical Bulletin·Koki OgawaShigeru Kawakami
Apr 27, 2020·Prenatal Diagnosis·Norman R Saunders, Katarzyna M Dziegielewska
Jun 9, 2018·Frontiers in Immunology·David A Menassa, Diego Gomez-Nicola
Jun 1, 2019·Annual Review of Nutrition·Kendra A TianiMartha S Field
Jul 9, 2020·International Journal of Molecular Sciences·Karol Kaiser, Vitezslav Bryja
Feb 19, 2016·Journal of Neuropathology and Experimental Neurology·Charanjit KaurEng-Ang Ling
May 26, 2018·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Tsutomu Nakada, Ingrid L Kwee
Mar 3, 2019·Journal of Neuroinflammation·Qianquan MaRong-Fu Wang
Oct 5, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Luke HarrisonPaul T Pfluger
Oct 14, 2016·Molecular BioSystems·Ronald ZielmanMichel D Ferrari
Jun 6, 2019·Brain Structure & Function·Daniela López-EspíndolaAna Guadaño-Ferraz
Oct 18, 2020·Pharmaceutics·Mark A BryniarskiMarilyn E Morris
Dec 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·David C Van Essen
Mar 27, 2021·Current Opinion in Neurology·Roland NauHelmut Eiffert
Jul 13, 2021·Frontiers in Molecular Biosciences·Kseniia Sarieva, Simone Mayer
Jul 22, 2021·Journal of Neuroscience Research·Omar Trujillo-CenózRaúl E Russo
Oct 12, 2021·Neuroscience Letters·Maurice PagninSamantha J Richardson

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE44072

Methods Mentioned

BETA
electron microscopy
dissecting
Fluorescence
RNA-Seq

Software Mentioned

Adobe Photoshop CC®
HTSeq
Ensembl
Bowtie
Bioconductor
ImageJ64
edgeR package

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.