The inner lives of focal adhesions
In focal adhesions of eukaryotic cells, transmembrane receptors of the integrin family and a large set of adaptor proteins form the physical link between the extracellular substrate and the actin cytoskeleton. During cell migration, nascent focal adhesions within filopodia and lamellipodia make the initial exploratory contacts with the cellular environment, whereas maturing focal adhesions pull the cell forward against the resistance of 'sliding' focal adhesions at the cell rear. Experimental approaches are now available for analysing the dynamics and interior structure of these different focal adhesions. Analysing focal-adhesion dynamics using green-fluorescent-protein-linked integrin leads us to propose that the acto-myosin-controlled density and turnover of integrins in focal adhesions is used to sense the elasticity and spacing of extracellular ligands, regulating cell migration by mechanically transduced signaling.
An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation
Fibronectin receptor exhibits high lateral mobility in embryonic locomoting cells but is immobile in focal contacts and fibrillar streaks in stationary cells.
Immunoelectron microscopic studies of the sites of cell-substratum and cell-cell contacts in cultured fibroblasts
Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
Dynamic localization of RNase MRP RNA in the nucleolus observed by fluorescent RNA cytochemistry in living cells
p140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin
Regulation of the association of adducin with actin filaments by Rho-associated kinase (Rho-kinase) and myosin phosphatase.
Rho-kinase in human neutrophils: a role in signalling for myosin light chain phosphorylation and cell migration
Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis
Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control-directed cell migration
EphA receptors regulate growth cone dynamics through the novel guanine nucleotide exchange factor ephexin
Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts
Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells
Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior
Invadopodia: specialized tumor cell structures for the focal degradation of the extracellular matrix
Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with components of the actin and intermediate filament cytoskeleton.
Novel p21-activated kinase-dependent protrusions characteristically formed at the edge of transformed cells
Lateral shear forces applied to cells with single elastic micropillars to influence focal adhesion dynamics
Global gene expression analysis identifies PDEF transcriptional networks regulating cell migration during cancer progression.
A direct interaction between the large GTPase dynamin-2 and FAK regulates focal adhesion dynamics in response to active Src
Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance
Clinical implications of microsomal prostaglandin e synthase-1 overexpression in human non-small-cell lung cancer
Astrocyte-derived tissue transglutaminase interacts with fibronectin: a role in astrocyte adhesion and migration?
Introducing dip pen nanolithography as a tool for controlling stem cell behaviour: unlocking the potential of the next generation of smart materials in regenerative medicine.
Assessing the spatial resolution of cellular rigidity sensing using a micropatterned hydrogel-photoresist composite
PyK2 and FAK connections to p190Rho guanine nucleotide exchange factor regulate RhoA activity, focal adhesion formation, and cell motility
New PI(4,5)P2- and membrane proximal integrin-binding motifs in the talin head control beta3-integrin clustering.
Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
How to awaken your nanomachines: Site-specific activation of focal adhesion kinases through ligand interactions
Paxillin and hydrogen peroxide-inducible clone 5 expression and distribution in control and Alzheimer disease hippocampi.
Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.
Adhesion Molecules in Health and Disease
Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.