The INO80 Complex Removes H2A.Z to Promote Presynaptic Filament Formation during Homologous Recombination

Cell Reports
Claudio A LademannStefan Jentsch

Abstract

The INO80 complex (INO80-C) is an evolutionarily conserved nucleosome remodeler that acts in transcription, replication, and genome stability. It is required for resistance against genotoxic agents and is involved in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR). However, the causes of the HR defect in INO80-C mutant cells are controversial. Here, we unite previous findings using a system to study HR with high spatial resolution in budding yeast. We find that INO80-C has at least two distinct functions during HR-DNA end resection and presynaptic filament formation. Importantly, the second function is linked to the histone variant H2A.Z. In the absence of H2A.Z, presynaptic filament formation and HR are restored in INO80-C-deficient mutants, suggesting that presynaptic filament formation is the crucial INO80-C function during HR.

Citations

Aug 30, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ashby J Morrison
Jun 22, 2019·Genetics·Ujani ChakrabortyEric Alani
May 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Panagiotis KarakaidosTheodoros Rampias
Sep 27, 2019·Frontiers in Molecular Biosciences·Susanne C S Bantele, Boris Pfander
Dec 30, 2017·Genes & Development·Michael H Hauer, Susan M Gasser
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chaoyou XueEric C Greene
Aug 14, 2020·Cells·Radoslav AleksandrovAnastas Gospodinov
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Jul 8, 2020·Seminars in Cell & Developmental Biology·Alexander Carver, Xiaodong Zhang
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Aug 1, 2019·Genetics·Ujani ChakrabortyEric Alani
Jun 20, 2021·Journal of Biomedical Science·Chia-Ling HsuCheng-Fu Kao

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