The inorganic pyrophosphate transporter ANK preserves the differentiated phenotype of articular chondrocyte.

The Journal of Biological Chemistry
Frederic CailottoArnaud Bianchi

Abstract

The differentiated phenotype of chondrocyte is lost in pathological situations and after interleukin (IL)-1beta challenge. Wnt proteins and the inorganic pyrophosphate (PP(i)) transporter Ank regulate the differentiation process in many cell types. We investigated the possible contribution of Ank and/or PP(i) to the maintenance of the differentiated chondrocyte phenotype with special care to Wnt signaling. Primary articular chondrocytes lost their phenotype upon IL-1beta challenge, with cessation of type II collagen and Sox-9 expression. Ank expression and PP(i) transport were strongly reduced by IL-1beta, whereas Wnt-5a was the only Wnt protein increased. Transient overexpression of Ank counteracted most of IL-1beta effects on Type II collagen, Sox-9, and Wnt-5a expression. When resting chondrocytes were transfected with a siRNA against Ank, this reproduced the phenotype induced by IL-1beta. In both cases, no markers for hypertrophic chondrocytes were detected. The conditioned supernatant from chondrocytes knocked-down for Ank contained Wnt-5a, which activated Tcf/Lef reporter plasmids and promoted translocation of beta-catenin into the nucleus without activating the c-Jun N-terminal kinase (JNK) pathway. Supplementation with ...Continue Reading

References

Dec 1, 1994·The Journal of Clinical Investigation·M B GoldringJ F Apperley
Oct 1, 1996·Current Biology : CB·J Yang-SnyderR T Moon
Aug 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Q ZhaoB De Crombrugghe
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Oct 20, 1999·Frontiers in Bioscience : a Journal and Virtual Library·J Martel-PelletierJ P Pelletier
Feb 1, 2000·The Journal of Biological Chemistry·S MurakamiB de Crombrugghe
Feb 5, 2000·Biochimica Et Biophysica Acta·M J Seidensticker, J Behrens
Sep 12, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B de CrombruggheW Huang
Mar 22, 2001·The New England Journal of Medicine·E H Choy, G S Panayi
Sep 5, 2001·Current Opinion in Genetics & Development·J Huelsken, W Birchmeier
Dec 26, 2001·EMBO Reports·Frank J T StaalHans C Clevers
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·T Aigner, L McKenna
Oct 31, 2002·Development·Je-Hwang RyuJang-Soo Chun
Jan 28, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Yingzi Yang
Dec 17, 2004·Molecular and Cellular Biology·Wei WangThorsten Kirsch
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Herman S Cheung
Jun 24, 2005·The American Journal of Pathology·Yukio NakamuraShigeyuki Wakitani
Aug 16, 2005·FEBS Letters·Sang-Gu HwangJang-Soo Chun
Oct 11, 2005·Journal of Cellular Biochemistry·Mary B GoldringKosei Ijiri
Feb 10, 2006·Calcified Tissue International·B L FosterM J Somerman
Jul 13, 2006·Annals of the New York Academy of Sciences·Maurizio PacificiMasahiro Iwamoto
Sep 19, 2006·Best Practice & Research. Clinical Rheumatology·Mary B Goldring
Oct 20, 2006·The Journal of Biological Chemistry·Simon R Tew, Timothy E Hardingham
Jun 30, 2007·Nature Clinical Practice. Rheumatology·Thomas AignerJochen Haag
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jochen ZwerinaGeorg Schett
Sep 4, 2008·Science Signaling·Renée van AmerongenRoel Nusse
Mar 17, 2009·Journal of Cellular Biochemistry·Tracy A DenisonBarbara D Boyan

❮ Previous
Next ❯

Citations

Mar 6, 2013·Nature Reviews. Rheumatology·Rik J LoriesNancy E Lane
Jul 7, 2014·Nature Medicine·Jean de la Croix NdongFlorent Elefteriou
Nov 30, 2013·Arthritis Research & Therapy·Ann K RosenthalLawrence M Ryan
Jan 29, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bo WuWei-Bo Xia
Apr 2, 2016·Current Rheumatology Reports·Elizabeth Mitton-FitzgeraldAnn K Rosenthal
Jul 7, 2010·The International Journal of Biochemistry & Cell Biology·Jessica BertrandThomas Pap
Feb 3, 2012·The Journal of Rheumatology·Facundo Las HerasFlorence W L Tsui

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.