The insecticidal activity of recombinant garlic lectins towards aphids.

Insect Biochemistry and Molecular Biology
Elaine FitchesJ A Gatehouse

Abstract

The heterodimeric and homodimeric garlic lectins ASAI and ASAII were produced as recombinant proteins in the yeast Pichia pastoris. The proteins were purified as functional dimeric lectins, but underwent post-translational proteolysis. Recombinant ASAII was a single homogenous polypeptide which had undergone C-terminal processing similar to that occurring in planta. The recombinant ASAI was glycosylated and subject to variable and heterogenous proteolysis. Both lectins showed insecticidal effects when fed to pea aphids (Acyrthosiphon pisum) in artificial diet, ASAII being more toxic than ASAI at the same concentration. Acute toxicity (mortality at < or =48 h exposure; similar timescale to starvation) was only apparent at the highest lectin concentrations tested (2.0 mg ml(-)1), but dose-dependent chronic toxicity (mortality at >3d exposure) was observed over the concentration range 0.125-2.0 mg ml(-1). The recombinant lectins caused mortality in both symbiotic and antibiotic-treated aphids, showing that toxicity is not dependent on the presence of the bacterial symbiont (Buchnera aphidicola), or on interaction with symbiont proteins, such as the previously identified lectin "receptor" symbionin. A pull-down assay coupled with p...Continue Reading

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Citations

Oct 5, 2010·Applied Microbiology and Biotechnology·Sze Kwan Lam, Tzi Bun Ng
Aug 27, 2011·Applied Microbiology and Biotechnology·Santosh Kumar UpadhyayPradhyumna Kumar Singh
May 25, 2012·The Protein Journal·Santosh K Upadhyay, Pradhyumna K Singh
Mar 5, 2011·PloS One·Gianni VandenborreEls J M Van Damme
Jun 29, 2012·Bioscience, Biotechnology, and Biochemistry·Koh AmanoYoshiho Nagata
Aug 21, 2015·Medicinal Research Reviews·Xiuli DanTzi Bun Ng
Apr 26, 2012·Critical Reviews in Biotechnology·Carla OliveiraLucília Domingues
May 18, 2016·Iranian Red Crescent Medical Journal·Mina Cheraghi NiroumandMohammad Reza Shams-Ardekani
Oct 13, 2015·The Journal of Organic Chemistry·Shu-Lun TangNicola L B Pohl
May 14, 2009·Pest Management Science·Shiang Jiuun ChenRong Nan Huang
Dec 20, 2014·Journal of Insect Science·Iwona SprawkaHubert Sytykiewicz
Jan 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Lígia R MacedoCarolina T Oliveira
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Jan 1, 2014·Insects·Margaret C Hardy
Feb 3, 2019·Plant Science : an International Journal of Experimental Plant Biology·Vamsi NalamJyoti Shah

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