The Insulator Protein CTCF Is Required for Correct Hox Gene Expression, but Not for Embryonic Development in Drosophila

Genetics
Maria Cristina Gambetta, Eileen E M Furlong

Abstract

Insulator binding proteins (IBPs) play an important role in regulating gene expression by binding to specific DNA sites to facilitate appropriate gene regulation. There are several IBPs in Drosophila, each defined by their ability to insulate target gene promoters in transgenic assays from the activating or silencing effects of neighboring regulatory elements. Of these, only CCCTC-binding factor (CTCF) has an obvious ortholog in mammals. CTCF is essential for mammalian cell viability and is an important regulator of genome architecture. In flies, CTCF is both maternally deposited and zygotically expressed. Flies lacking zygotic CTCF die as young adults with homeotic defects, suggesting that specific Hox genes are misexpressed in inappropriate body segments. The lack of any major embryonic defects was assumed to be due to the maternal supply of CTCF protein, as maternally contributed factors are often sufficient to progress through much of embryogenesis. Here, we definitively determined the requirement of CTCF for developmental progression in Drosophila We generated animals that completely lack both maternal and zygotic CTCF and found that, contrary to expectation, these mutants progress through embryogenesis and larval life. Th...Continue Reading

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Citations

Sep 29, 2018·Science·Eileen E M Furlong, Michael Levine
Mar 20, 2019·Nature Reviews. Molecular Cell Biology·Bas van Steensel, Eileen E M Furlong
Mar 20, 2019·Nature·Leslie J MateoAlistair N Boettiger
May 31, 2019·Genes·Irene Mota-Gómez, Darío G Lupiáñez
Jul 3, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nicholas E Matthews, Rob White
Oct 2, 2019·Genes·Isa Özdemir, Maria Cristina Gambetta
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Apr 17, 2019·Science Advances·Quentin SzaboGiacomo Cavalli
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Sep 28, 2021·G3 : Genes - Genomes - Genetics·Shreekant VermaRakesh K Mishra

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Methods Mentioned

BETA
PCR
Hi-C
transgenic

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