The insulin receptor substrate 1 (IRS1) in intestinal epithelial differentiation and in colorectal cancer.

PloS One
Diana L EspositoRenato Mariani-Costantini

Abstract

Colorectal cancer (CRC) is associated with lifestyle factors that affect insulin/IGF signaling, of which the insulin receptor substrate 1 (IRS1) is a key transducer. We investigated expression, localization and pathologic correlations of IRS1 in cancer-uninvolved colonic epithelium, primary CRCs with paired liver metastases and in vitro polarizing Caco2 and HT29 cells. IRS1 mRNA and protein resulted higher, relative to paired mucosa, in adenomas of familial adenomatous polyposis patients and in CRCs that overexpressed c-MYC, ß-catenin, InsRß, and IGF1R. Analysis of IRS1 immunostaining in 24 cases of primary CRC with paired colonic epithelium and hepatic metastasis showed that staining intensity was significantly higher in metastases relative to both primary CRC (P<0.01) and colonic epithelium (P<0.01). Primary and metastatic CRCs, compared to colonic epithelium, contained significantly higher numbers of IRS1-positive cells (P = 0.013 and P = 0.014, respectively). Pathologic correlations in 163 primary CRCs revealed that diffuse IRS1 staining was associated with tumors combining differentiated phenotype and aggressive markers (high Ki67, p53, and ß-catenin). In Caco 2 IRS1 and InsR were maximally expressed after polarization, wh...Continue Reading

References

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·M RotschK Havemann
Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M IlyasW F Bodmer
Feb 26, 2000·Carcinogenesis·S P BachC S Potten
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·A J RowanI P Tomlinson
Jul 4, 2002·Journal of the National Cancer Institute·Manjinder S SandhuEdward L Giovannucci
Aug 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Morris F White
Oct 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Niels ReinmuthLee M Ellis
Jan 8, 2004·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·X TuR Baserga
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert DrakasRenato Baserga
Jan 15, 2005·International Journal of Colorectal Disease·Rajaraman DuraiMarc C Winslet
Jun 14, 2005·Cancer Cell·Yardena SamuelsVictor E Velculescu
May 26, 2006·Nature·Reuben J Shaw, Lewis C Cantley
Jun 9, 2006·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F BishehsariUNKNOWN Gruppo Oncologico dell'Italia Meridionale
Apr 12, 2007·Science's STKE : Signal Transduction Knowledge Environment·Coralie A Carothers Carraway, Kermit L Carraway
Jun 27, 2007·Alimentary Pharmacology & Therapeutics·I T Johnson, E K Lund
Sep 8, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·J G SimmonsP K Lund
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lanlan ShenJean-Pierre J Issa
Nov 26, 2008·Nature Reviews. Cancer·Michael Pollak

❮ Previous
Next ❯

Citations

Dec 29, 2012·Current Diabetes Reports·Etan Orgel, Steven D Mittelman
May 25, 2013·European Journal of Medicinal Chemistry·Simone CarradoriBruna Bizzarri
Nov 28, 2012·Clinical & Experimental Metastasis·A A P SlesserP Tekkis
Nov 4, 2015·Frontiers in Oncology·Paolo Giovanni VigneriLivia Manzella
Apr 4, 2017·International Journal of Cancer. Journal International Du Cancer·Marta SchirripaHeinz-Josef Lenz
Jan 22, 2018·Journal of Gastroenterology·Patricia Sanmartín-SalinasLuis Gonzalez Guijarro
May 26, 2018·Annual Review of Nutrition·Salvador Alonso, Ömer H Yilmaz
Nov 13, 2019·Journal of Cellular Biochemistry·Mei TangHui Zhang
Dec 2, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tasha BarrIlhem Messaoudi
Nov 19, 2015·Annals of Surgical Oncology·Akiko HanyudaShuji Ogino
May 20, 2020·Bioinformatics and Biology Insights·Badreddine NouadiFatima Chegdani
Jul 24, 2013·Oncology Reports·Diana Liberata EspositoAlessandro Cama
Feb 12, 2014·Molecular Biology Reports·Yong LiDong Wang
Aug 22, 2020·World Journal of Gastroenterology : WJG·Karolina LompertaMariusz Koda
Dec 1, 2017·Journal of Molecular Histology·Patricia Sanmartín-SalinasLuis G Guijarro
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Aneesha Kulkarni, Laura W Bowers
Jan 6, 2021·Cells·Crescenzo MassaroCarmela Dell'Aversana
Apr 9, 2021·Nutrition & Metabolism·Katayoun Pourvali, Hadi Monji

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ubiquitination
biopsy
transmission electron microscopy

Software Mentioned

AxioVision
SPSS
Primer Express
ImageJ

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.