The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway

Life Sciences
Soo Bong ChoiSunmin Park

Abstract

In the present study, we screened candidates for enhancing insulin action, using glucose uptake as an indicator, from Liriope platyphylla Wang et Tang (LPWT) extract, Liliaceae, in 3T3-L1 adipocytes. The mechanism of insulin sensitizing action in the fractions was also investigated. LPWT extract with 70% MeOH was sequentially separated with Diaion HP-20 and silica gel column chromatography. The 9:1 fraction from silica gel column chromatography increased glucose uptake with 1 ng/mL up to glucose uptake with 50 ng/mL insulin. The 9:1 fraction, determined as homoisoflavone-enriched fraction, worked as an insulin sensitizer. It increased insulin stimulated glucose uptake in 3T3-L1 adipocytes, insulin responsive cells, through increased glucose transporter 4 (GLUT4) contents in the plasma membrane. GLUT4 translocation was increased through insulin receptor substrate 1 (IRS1)-PI3 kinase-Akt signaling mechanism. Thus, homoisoflavone-enriched fraction in LPWT extract played an important role as an insulin sensitizer in adipocytes.

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Citations

Aug 16, 2013·Journal of Asian Natural Products Research·Yunkyung HanYong-Ki Park
Nov 28, 2013·BMC Complementary and Alternative Medicine·Ji Eun KimDae Youn Hwang
Aug 5, 2005·Biological & Pharmaceutical Bulletin·Byoung-Seob KoSunmin Park
Feb 14, 2016·Life Sciences·Fatma HadrichSami Sayadi
Mar 13, 2016·Journal of Ethnopharmacology·Hyun Jung KimByung Joo Kim
Jun 25, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kum-Ju ParkChang-Kee Hyun
Jul 4, 2015·Natural Product Research·Salar Hafez GhoranHidayat Hussain
Sep 16, 2016·The American Journal of Chinese Medicine·Min-Jee KimMee Ree Kim
Jul 6, 2021·Biochemistry Research International·Molly MombeshoraStanley Mukanganyama

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