The integrase of the Macrococcus caseolyticus resistance island mecD (McRImecD ) inserts DNA site-specifically into Staphylococcus and Bacillus chromosomes

Molecular Microbiology
Sybille Schwendener, Vincent Perreten

Abstract

The methicillin resistance gene mecD has been recently identified on chromosomal islands in Macrococcus caseolyticus (McRImecD ). The 5' end of McRImecD carries an integrase (int) of the tyrosine recombinase family and two genes (intR and xis) encoding putative DNA-binding proteins. The islands are integrated site-specifically at the 3' end of the rpsI gene, a highly conserved locus in Gram-positive bacteria. Moreover, the rpsI gene of some Staphylococcus and Bacillus strains was found to be followed by a related integrase, raising the question of whether McRImecD could be transferred to these species. We used circular model elements carrying 5' end fragments of McRImecD -1 to demonstrate that the int enzyme and the attachment (att) site were sufficient to mediate site-specific DNA integration into the rpsI locus of Staphylococcus aureus, Staphylococcus pseudintermedius and Bacillus thuringiensis in vivo. Including xis in the model element stimulated both integrative and excisive recombination reactions and influenced the Int enzyme in att site selection. The intR gene functions as a negative regulator of int and xis. The int-xis genes of McRImecD -1 encode a site-specific recombination function that enables the acquisition of ...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L Moitoso de Vargas, A Landy
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S YinA Landy
Jun 1, 1997·FEMS Microbiology Letters·R Brückner
Sep 15, 1997·Nucleic Acids Research·D Esposito, J J Scocca
Feb 13, 2003·Journal of Molecular Biology·John A Lewis, Graham F Hatfull
Jun 25, 2004·Antimicrobial Agents and Chemotherapy·Teruyo ItoKeiichi Hiramatsu
Mar 31, 2005·Current Opinion in Genetics & Development·Ian B DoddJ Barry Egan
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice
Jun 5, 2007·Journal of Bacteriology·Elisa MaiquesJosé R Penadés
Jan 21, 2010·Antimicrobial Agents and Chemotherapy·Sae TsubakishitaKeiichi Hiramatsu
May 18, 2010·Nature·María Angeles Tormo-MásJosé R Penadés
Jul 17, 2010·Nature Reviews. Microbiology·Richard P NovickJose R Penadés
Jun 30, 2012·Molecular Microbiology·Ignacio Mir-SanchisJosé R Penadés
Mar 19, 2013·International Journal of Medical Microbiology : IJMM·Anna C Shore, David C Coleman
Jul 4, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Natacha CoutoConstança Pomba
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Mar 14, 2016·Trends in Microbiology·Nadine FornelosMatej Butala
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

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