The integration of LC-MS and NMR for the analysis of low molecular weight trace analytes in complex matrices.

Mass Spectrometry Reviews
Rose M GathunguPaul Vouros

Abstract

This review discusses the integration of liquid chromatography (LC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) in the comprehensive analysis of small molecules from complex matrices. We first discuss the steps taken toward making the three technologies compatible, so as to create an efficient analytical platform. The development of online LC-MS-NMR, highlighted by successful applications in the profiling of highly concentrated analytes (LODs 10 μg) is discussed next. This is followed by a detailed overview of the alternative approaches that have been developed to overcome the challenges associated with online LC-MS-NMR that primarily stem from the inherently low sensitivity of NMR. These alternative approaches include the use of stop-flow LC-MS-NMR, loop collection of LC peaks, LC-MS-SPE-NMR, and offline NMR. The potential and limitations of all these approaches is discussed in the context of applications in various fields, including metabolomics and natural product discovery.

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Citations

Jun 17, 2020·Expert Review of Neurotherapeutics·Roberta BonomoDebra J Skene
Nov 6, 2018·Frontiers in Endocrinology·Tristan StrujaPhilipp Schuetz
Jan 17, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Barbara Pacholczyk-SienickaŁukasz Albrecht
Nov 24, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiaojiao ChenXiufeng Ling
Apr 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuanchen LiuYusuke Yamauchi
Aug 23, 2021·EBioMedicine·Jacob T BjerrumOle H Nielsen

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