After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. This 'revolution in resolution' is happening largely thanks to new developments of new-generation cameras used for recording the images in the cryo electron microscope which have much increased sensitivity being based on complementary metal oxide semiconductor devices. Combined with advanced image processing and 3D reconstruction, the cryo-EM analysis of nucleoprotein complexes can provide unprecedented insights at molecular and atomic levels and address regulatory mechanisms in the cell. These advances reinforce the integrative role of cryo-EM in synergy with other methods such as X-ray crystallography, fluorescence imaging or focussed-ion beam milling as exemplified here by some recent studies from our laboratory on ribosomes, viruses, chromatin and nuclear receptors. Such multi-scale and multi-resolution approaches allow integrating molecular and cellular levels when applied to purified or in situ macromolec...Continue Reading
Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy
Recognition and separation of single particles with size variation by statistical analysis of their images
Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset
A method of focused classification, based on the bootstrap 3D variance analysis, and its application to EF-G-dependent translocation
Imaging of organelles by electron microscopy reveals protein-protein interactions in mitochondria and chloroplasts
Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions.
In vivo virus structures: simultaneous classification, resolution enhancement, and noise reduction in whole-cell electron tomography
Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging.
Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER.
A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography
Single particle and molecular assembly analysis of polyribosomes by single- and double-tilt cryo electron tomography
Advances, interactions, and future developments in the CNS, Phenix, and Rosetta structural biology software systems.
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM
Automated target segmentation and real space fast alignment methods for high-throughput classification and averaging of crowded cryo-electron subtomograms
Cryo-electron microscopy and X-ray crystallography: complementary approaches to structural biology and drug discovery
3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations
Deriving and refining atomic models in crystallography and cryo-EM: the latest Phenix tools to facilitate structure analysis
Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure
Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.
Pathogenic missense protein variants affect different functional pathways and proteomic features than healthy population variants.
Carcinoma, Squamous Cell
Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.