The integrin-binding protein Nischarin regulates cell migration by inhibiting PAK

The EMBO Journal
Suresh K AlahariRudy L Juliano

Abstract

Nischarin, a novel intracellular protein, was originally identified as a binding partner for the alpha5beta1 integrin. Here we show that Nischarin also interacts with members of the PAK family of kinases. The amino terminus of Nischarin preferentially binds to the carboxy-terminal domain of PAK1 when the kinase is in its activated conformation. Nischarin binding to PAK1 is enhanced by active Rac, with the three proteins forming a complex, while expression of the alpha5beta1 integrin also increases the Nischarin/PAK1 association. Interaction with Nischarin strongly inhibits the ability of PAK1 to phosphorylate substrates. This effect on PAK kinase activity closely parallels Nischarin's ability to inhibit cell migration. Conversely, reduction of endogenous levels of Nischarin by RNA interference promotes cell migration. In addition, PAK1 and Nischarin colocalize in membrane ruffles, structures known to be involved in cell motility. Thus, Nischarin may regulate cell migration by forming inhibitory complexes with PAK family kinases.

References

Aug 30, 1996·The Journal of Biological Chemistry·M L GalisteoJ Schlessinger
Oct 18, 1996·The Journal of Biological Chemistry·G M BokochU G Knaus
Mar 1, 1997·Current Biology : CB·M A SellsJ Chernoff
Mar 26, 1999·Science·L C SandersP de Lanerolle
May 20, 1999·The Journal of Cell Biology·M A SellsJ Chernoff
Aug 26, 1999·Trends in Cell Biology·S Bagrodia, R A Cerione
Nov 24, 1999·The Journal of Biological Chemistry·M F CarlierD Pantaloni
May 19, 2000·The Biochemical Journal·A L Bishop, A Hall
Nov 18, 2000·Experimental Cell Research·S K Sastry, K Burridge
Jan 3, 2001·The Journal of Cell Biology·M A SellsJ Chernoff
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·C XiaM Liu
Jul 5, 2001·Molecular and Cellular Biology·G BuchwaldA Wittinghofer
Dec 26, 2001·The Journal of Biological Chemistry·Mengwei ZangZhijun Luo
Jan 5, 2002·The Journal of Cell Biology·M E Hemler
Jan 5, 2002·Molecular and Cellular Biology·Chuntao DanAudrey Minden
Mar 26, 2002·The Journal of Biological Chemistry·Hiroyuki SanoGustav E Lienhard
Aug 29, 2002·Nature Cell Biology·Ratna K VadlamudiRakesh Kumar
Sep 11, 2002·Molecular Biology of the Cell·Smita AbbiJun-Lin Guan
Sep 13, 2002·International Review of Cytology·Suresh K AlahariR L Juliano
Oct 18, 2002·Journal of Cellular Physiology·Rakesh Kumar, Ratna K Vadlamudi
Oct 31, 2002·Pharmaceutical Research·Dongjiu YeR L Juliano
Jan 11, 2003·The Journal of Biological Chemistry·Lorena A PutoGary M Bokoch
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Apr 5, 2003·Annual Review of Biochemistry·Gary M Bokoch

❮ Previous
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Citations

Sep 26, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xiaohong WuXingxing Gu
Jan 24, 2009·Cancer Metastasis Reviews·Bettina DummlerJeffrey Field
May 26, 2006·Nature Reviews. Cancer·Rakesh KumarChristopher J Barnes
May 26, 2009·Oncogene·P R MolliR Kumar
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rajesh GhaiBrett M Collins
Nov 5, 2011·The Journal of Biological Chemistry·Geok-Lin ChuaSurajit Bhattacharjya
Jan 13, 2009·The Journal of Biological Chemistry·Chunming ChengJianxin Gu
Sep 16, 2011·Journal of the National Cancer Institute·Somesh BaranwalSuresh K Alahari
Mar 12, 2008·Molecular and Cellular Biology·Yuemin DingSuresh K Alahari
Jun 1, 2005·Molecular and Cellular Biology·Ashok PullikuthAndrew D Catling
May 19, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeyanthy EswaranRakesh Kumar
Jan 26, 2012·BMC Medical Genetics·Natalia Briones, Valentin Dinu
Jul 31, 2012·PloS One·Geok-Lin ChuaSurajit Bhattacharjya
May 7, 2011·Theranostics·Rudy L JulianoHoon Yoo
Jul 24, 2014·BMC Genomics·Vincent GrassotJean-Michel Petit
Feb 12, 2010·Expert Opinion on Therapeutic Targets·Xiaodong LiFeng Li
Feb 22, 2007·The Journal of Cell Biology·A Wayne OrrMartin Alexander Schwartz
Feb 20, 2015·Molecular Medicine Reports·Jie ChenShang-Nao Xie
Jul 22, 2008·Trends in Biochemical Sciences·Jeyanthy EswaranStefan Knapp
Jun 9, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Martina NagelRudolf Winklbauer
Sep 3, 2011·Cardiovascular Therapeutics·Lincoln P EdwardsPaul Ernsberger
May 6, 2014·Journal of Ethnopharmacology·Peiwei WangTeng Zhang
Nov 6, 2012·Cellular Logistics·Maria Carla Parrini
Jan 6, 2009·Biochemical and Biophysical Research Communications·Benjamin MentzelThomas Raabe
Aug 30, 2016·ACS Chemical Biology·Lewis H MervinAndreas Bender
Dec 23, 2016·Gene·Rakesh KumarFeng Li
Aug 15, 2017·PLoS Genetics·Michael CromptonSteve D M Brown
Feb 9, 2018·Molecular Cancer·Mazvita MaziveyiSuresh K Alahari
Apr 15, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Olivia LuuRudolf Winklbauer
Nov 10, 2005·Oncogene·A H TalukderR Kumar
Jul 18, 2019·Physiological Reviews·Michael BachmannBernhard Wehrle-Haller
Dec 26, 2013·American Journal of Physiology. Endocrinology and Metabolism·Yu-ting Alex Chiang, Tianru Jin
Sep 17, 2019·International Journal of Cancer. Journal International Du Cancer·Shengli DongSuresh K Alahari

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