The interaction between (Ca2+ + Mg2+)-ATPase and the soluble activator (calmodulin) in erythrocytes containing haemoglobin S

British Journal of Haematology
E Dixon, R M Winslow

Abstract

In normal erythrocytes, a membrane-bound (Ca2+ + Mg2+)-ATPase is stimulated by a soluble activator, calmodulin. Since cells containing Hb S accumulate excessive Ca2+, the defect could lie in either the (Ca2+ + Mg2+)-ATPase or calmodulin. To decide between these two possibilities, we prepared (Ca2+ + Mg2+)-ATPase from erythrocytes of normal (AA), sickle cell trait (AS) and sickle cell disease (SS) individuals. Calmodulin was prepared from haemolysates from AA and SS erythrocytes. The enzyme prepared from SS ghosts had lower specific activity than that from AA membranes. Furthermore, calmodulin from either source did not stimulate the ATPase of SS erythrocytes. Enzyme from AS cells had specific activity similar to that of enzyme prepared from SS membranes. The enzymatic activity of a mixed cell population obtained from an SS patient 8 d following exchange-transfusion was proportional to the per cent Hb A. These results indicate that calmodulin is unable to interact with the enzyme site on the SS membrane. This inability is believed to be due to a specific property of the membrane and not an abnormality of calmodulin itself.

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Citations

Jan 1, 1982·Pharmacology & Therapeutics·J S Wiley, K E McCulloch
Dec 1, 1982·The Journal of Clinical Investigation·R P HebbelM H Steinberg
Jan 1, 1983·Annals of the New York Academy of Sciences·F J Nordt
Sep 1, 1983·American Journal of Hematology·J A WarthK Near
Feb 1, 1990·Biochemical Medicine and Metabolic Biology·E Friederichs, A Würz

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