The interaction between circulating complement proteins and cutaneous microvascular endothelial cells in the development of childhood Henoch-Schönlein Purpura

PloS One
Yao-Hsu YangBor-Luen Chiang

Abstract

In addition to IgA, the deposition of complement (C)3 in dermal vessels is commonly found in Henoch-Schönlein purpura (HSP). The aim of this study is to elucidate the role of circulating complement proteins in the pathogenesis of childhood HSP. Plasma levels of C3a, C4a, C5a, and Bb in 30 HSP patients and 30 healthy controls were detected by enzyme-linked immunosorbent assay (ELISA). The expression of C3a receptor (C3aR), C5a receptor (CD88), E-selectin, intercellular adhesion molecule 1 (ICAM-1), C3, C5, interleukin (IL)-8, monocyte chemotactic protein (MCP)-1, and RANTES by human dermal microvascular endothelial cells (HMVEC-d) was evaluated either by flow cytometry or by ELISA. At the acute stage, HSP patients had higher plasma levels of C3a (359.5 ± 115.3 vs. 183.3 ± 94.1 ng/ml, p < 0.0001), C5a (181.4 ± 86.1 vs. 33.7 ± 26.3 ng/ml, p < 0.0001), and Bb (3.7 ± 2.6 vs. 1.0 ± 0.6 μg/ml, p < 0.0001), but not C4a than healthy controls. Although HSP patient-derived acute phase plasma did not alter the presentation of C3aR and CD88 on HMVEC-d, it enhanced the production of endothelial C3 and C5. Moreover, C5a was shown in vitro to up-regulate the expression of IL-8, MCP-1, E-selectin, and ICAM-1 by HMVEC-d with a dose-dependent man...Continue Reading

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Citations

Nov 27, 2015·Molecular and Cellular Neurosciences·K Sue O'Shea, Melvin G McInnis
Nov 27, 2019·Rheumatology·Shirly FrizinskyNancy Agmon-Levin
Sep 14, 2018·Acta Neuropathologica Communications·T A M Bouwens van der VlisP J van der Spek
Nov 4, 2020·Immunity, Inflammation and Disease·Bo LiKai Le
Jun 3, 2021·Journal of Clinical Medicine·Evangeline Pillebout
Jul 25, 2021·International Journal of Molecular Sciences·Hitomi SuginoMotonobu Nakamura

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Methods Mentioned

BETA
biopsies
enzyme-linked immunosorbent assay
ELISA
flow cytometry

Software Mentioned

CellQuest

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