The interaction between miR160 and miR165/166 in the control of leaf development and drought tolerance in Arabidopsis

Scientific Reports
Tianxiao YangGuiliang Tang

Abstract

MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development and abiotic stresses. To date, studies have mainly focused on the roles of individual miRNAs, however, a few have addressed the interactions among multiple miRNAs. In this study, we investigated the interplay and regulatory circuit between miR160 and miR165/166 and its effect on leaf development and drought tolerance in Arabidopsis using Short Tandem Target Mimic (STTM). By crossing STTM160 Arabidopsis with STTM165/166, we successfully generated a double mutant of miR160 and miR165/166. The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines. RNA-seq and qRT-PCR analyses suggested that the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants. Our results also suggested that miR160-directed regulation of auxin response factors (ARFs) contribute to leaf development via auxin signaling genes, whereas miR165/166- mediated HD-ZIP IIIs regulation confers drought tolerance through ABA signaling. Our studies further indicated that ARFs an...Continue Reading

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Citations

Sep 8, 2019·Viruses·Baogang ZhangJianguo Wu
Apr 1, 2020·International Journal of Molecular Sciences·Barbara WójcikowskaMałgorzata D Gaj
Aug 20, 2020·Plant Molecular Biology·Melisa LeoneSebastián Asurmendi
Apr 23, 2020·International Journal of Molecular Sciences·Cheng-Wei QiuFeibo Wu
Nov 5, 2019·Frontiers in Plant Science·Xuedong LiuTao Zhou
Jul 24, 2020·International Journal of Molecular Sciences·Shahid AliLinan Xie
Oct 18, 2020·International Journal of Molecular Sciences·Jack ColicchioLena Hileman
Dec 18, 2020·International Journal of Molecular Sciences·Na LiZhanhui Zhang
Apr 4, 2021·International Journal of Molecular Sciences·Zaira M López-JuárezPlinio Guzmán
May 7, 2021·Physiologia Plantarum·Garima SingrohaRamanjulu Sunkur
Jun 3, 2021·International Journal of Molecular Sciences·Carlos Lopez-OrtizUmesh K Reddy

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Methods Mentioned

BETA
transgenic
transgenics
RNA-seq
sRNA-seq
dissection
PCR
electrophoresis

Software Mentioned

SOAP2
DESeq
Target Mimicry ( TM
TopHat2
STTM
psRNATarget

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