The interaction between osteoclast-like cells and osteoblasts mediated by nanophase calcium phosphate-hybridized tendons

Biomaterials
Hirotaka MutsuzakiNaoyuki Ochiai

Abstract

We developed a novel technique of hybridizing calcium phosphate (CaP) with bioorganic soft tissue using an alternating soaking process. By this technique, we hybridized CaP with a grafted tendon tissue to bond with a bone tunnel. Tendons were soaked in Ca and NaHPO(4) solutions alternately for 10 min. Needle-like CaP crystals 30-50 nm in length including low-crystalline apatite were deposited on and between collagen fibrils from the surface to 200 microm deep in the tendon. In light and transmission electron microscopic images, osteoclast-like cells and osteoblasts appeared on the implanted tendon and osteoid was observed on the tendon surface at 1 week postoperatively. At 2 weeks postoperatively, osteoclast-like cells resolved the tendon by forming Howship's lacuna-like spaces on the surfaces and osteoblasts formed osteoid in these spaces. Direct bonding between the implanted tendon and the newly formed bone was observed. At 3 weeks postoperatively, thick newly formed bone firmly bonded to tendon surface. From these results, we conclude that the tendons prepared by an accelerated CaP hybridization method efficiently enhance osteoclast-like cells and osteoblasts to bond the implanted tendons to newly formed bone.

References

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Citations

Nov 8, 2008·Nature Nanotechnology·Y Shona PekJackie Y Ying
Dec 15, 2011·Sports Medicine, Arthroscopy, Rehabilitation, Therapy & Technology : SMARTT·Hirotaka Mutsuzaki, Masataka Sakane
Jan 24, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Masayuki TairaYoshima Araki
Apr 1, 2015·Journal of Shoulder and Elbow Surgery·Yoshiaki ItoigawaEiji Itoi
Jun 21, 2012·The American Journal of Sports Medicine·Hirotaka MutsuzakiMasataka Sakane
Sep 24, 2016·Orthopaedic Journal of Sports Medicine·Hirotaka MutsuzakiMasataka Sakane
Jul 28, 2017·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Hirotaka MutsuzakiMasataka Sakane

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