The interaction mechanism between fludarabine and human serum albumin researched by comprehensive spectroscopic methods and molecular docking technique

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
XiaoLe HanTao Huang

Abstract

Fludarabine (Flu) is widely used to treat B-cell chronic lymphocytic leukemia. HSA is of the essence to human, especially in blood circulation system. The interaction mechanism between Flu and HSA was studied by comprehensive spectroscopic methods and molecular docking technique. UV-vis and FL spectrum results indicated that Flu bond with HSA, and there was a new complex produced at the binding site I in subdomain IIA. Association constants at 298 K were 1.637 × 104 M-1 and 1.552 × 104 M-1 at 310 K, respectively. The negative enthalpy (ΔH) and positive entropy (ΔS) values for the interaction revealed that the binding behavior was driven by hydrophobic forces and hydrogen bonds. The results obtained from UV, RLS spectra, 3D fluorescence and CD spectrum illustrated that Flu could change the secondary structure of HSA. According to molecule docking result, the binding energy of interaction is -11.15 kcal/mol.

Citations

Jan 19, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sujuan ZhuBing Wang
Jul 18, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaolan LvXun Tuo
Aug 30, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Na LiXun Tuo

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