PMID: 4898917Oct 1, 1969Paper

The interaction of aflatoxin B1 with polynucleotides and its effect on ribonucleic acid polymerase

The Biochemical Journal
A M King, B H Nicholson

Abstract

1. The interaction of aflatoxin B(1) with different polynucleotides was studied spectrophotometrically. Equations were derived that enable the degree of binding to be determined without first determining the extinction coefficient of the bound form. 2. The interaction with calf thymus DNA obeys first-order relationships with an association constant of 0.40mm(-1), but there is some evidence for a secondary binding process from results obtained at 390nm. 3. The spectral shifts decreased in the order polyadenylic acid+polyuridylic acid>DNA>polyadenylic acid>polyadenylic acid+polyinosinic acid. Polycytidylic acid, polyuridylic acid, polyinosinic acid (both single- and triple-stranded), AMP, CMP, GMP and UMP did not interact with aflatoxin. It was concluded that there is a requirement for the amino group of adenine (or possibly guanine) for binding of aflatoxin to polynucleotides to occur. 4. Binding is reversed by increasing ionic strength, and by Mn(2+) and Mg(2+) in the concentration range studied (0-5mm). The effect of the Mn(2+) or Mg(2+) was far greater than would be expected on the basis of their ionic strength. With both the bivalent cations and sodium chloride the reversal is greatest with double-stranded polynucleotides. 5...Continue Reading

Citations

Jan 17, 2017·Journal of Biomolecular Structure & Dynamics·Fatemeh Khosravi, Hassan Mansouri-Torshizi
Sep 1, 1976·Journal of the American Oil Chemists' Society·P WellsR B Alfin-Slater
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Oct 30, 1970·Annals of the New York Academy of Sciences·G N Wogan, R S Pong
Jul 26, 2011·Nucleosides, Nucleotides & Nucleic Acids·H Mansouri-TorshiziA A Saboury
Oct 1, 1990·Cell Biology and Toxicology·M N BlaudeM B Roberfroid
Nov 24, 2011·Bioinorganic Chemistry and Applications·Hassan Mansouri-TorshiziFatemeh Hassani
Jun 8, 2013·Nucleosides, Nucleotides & Nucleic Acids·Maryam SaeidifarAli Akbar Saboury
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