The interaction of Agrobacterium Ti-plasmid DNA and plant cells

Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character
M Van MontaguJ Schell

Abstract

The tumour-inducing plasmids of Agrobacterium tumefaciens (Ti-plasmids) reveal several interesting properties. They are catabolic plasmids, which, instead of rendering Agrobacterium strains capable of catabolizing compounds found in Nature, force a plant to synthesize these catabolites (denoted 'opines'). This situation is obtained by insertion of a segment of the Ti-plasmid (the T-DNA) into the plant nucleus, where T-DNA genes become expressed and intervene in the biosynthesis of these opines. Cells containing the T-DNA behave as neoplasms (crown gall cells). Southern blotting shows that the insertion process responsible for T-DNA transfer probably recognizes special sequences on the T-DNA since the length of the T-DNA segment observed in different, independently isolated tumour lines was found to be similar. For the nopaline Ti-plasmids both left-hand and right-hand borders were found to be constant. For the octopine plasmid the left border was constant and at least two classes of right-hand borders were found. Upon redifferentiation of the transformed plant cells, the T-DNA was found to be conserved in all somatic cells examined. However, small deletions at the border fragments of the T-DNA have been observed. The exact arra...Continue Reading

Citations

Jan 1, 1983·Biotechnology Advances·J J PasternakB R Glick
Feb 1, 1982·Plant Physiology·R M Amasino, C O Miller
Mar 14, 2013·FEMS Microbiology Letters·Elisabeth StesDanny Vereecke
Aug 21, 2003·Trends in Plant Science·Matthew A Escobar, Abhaya M Dandekar
Jul 1, 1985·International Journal of Cell Cloning·W ScheithauerG Grabner
Nov 28, 2019·The New Phytologist·Marion O Harris, Andrea Pitzschke
Apr 5, 2020·Toxins·Hélène Bierne, Renaud Pourpre

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