The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme

Biochemistry
E K Hodgson, I Fridovich

Abstract

Bovine erythrocyte superoxide dismutase was slowly and irreversibly inactivated by hydrogen peroxide. The rate of this inactivation was directly dependent upon the concentrations of both H2O2 and of enzyme, and its second-order rate constant at pH 10.0 and 25 degrees was 6.7 M-1 sec-1. Inactivation was preceded by a bleaching due to rapid reduction of Cu2+ on the enzyme, and following this there was a gradual reappearance of a new absorption in the visible region, which was coincident with the loss of catalytic activity. Inactivation of the enzyme was pH-dependent and indicated an essential ionization whose pKa was approximately 10.2. Replacement of H2O by D2O raised this pKa but did not diminish the catalytic activity of superoxide dismutase, measured at pH 10.0. Several compounds, including xanthine, urate, formate, and azide, protected the enzyme against inactivation by H2O2. Alcohols and benzoate, which scavenge hydroxyl radical, did not protect. Compounds with special affinity for singlet oxygen were similarly ineffective. The data were interpreted in terms of the reduction of the enzyme-bound Cu2+ to Cu+, by H2O2, followed by a Fenton's type reaction of the Cu+ with additional H2O2. This would generate Cu2+-OH- or its ion...Continue Reading

Citations

Jan 7, 2000·Biochemical and Biophysical Research Communications·P PietrangeliL Morpurgo
Jan 1, 1991·Archives of Dermatological Research·Y HashimotoH Iizuka
Dec 1, 1988·Molecular and Cellular Biochemistry·D V GodinA D Goumeniouk
Mar 9, 1995·Molecular and Cellular Biochemistry·A K Singh, S Gulati
Jun 1, 1986·Journal of Neurocytology·L G ThaeteS S Spicer
Sep 8, 1993·Molecular and Cellular Biochemistry·G S DhaunsiC D Hanevold
Sep 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P C PanusB A Freeman
Jan 1, 1995·Folia Microbiologica·G Gille, K Sigler
Jul 2, 2003·Neurotoxicity Research·M B YimE R Stadtman
May 1, 1997·Journal of Neurology·M E Gurney
Jun 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S MehdizadehA J Lusis
Nov 6, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Melissa M PageJeffrey A Stuart
Apr 1, 1998·Age·M E GurneyD A Becker
Apr 1, 1998·Age·M B YimE R Stadtman
Jan 30, 1978·Archives of Biochemistry and Biophysics·S Kanematsu, K Asada
Dec 8, 1994·Biochimica Et Biophysica Acta·G M Wilkins, D S Leake
Jan 5, 1996·Chemico-biological Interactions·L H ChenD A Cohen
Oct 14, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·J C KoningsbergerJ J Marx
Apr 2, 1987·Journal of Immunological Methods·M J ArthurR Wright
Dec 1, 1984·Mechanisms of Ageing and Development·S ImreJ Fachet
May 1, 1987·Mechanisms of Ageing and Development·S Imre, E Juhász

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.