The interaction of C.I. acid red 27 with human hemoglobin in solution

Journal of Photochemistry and Photobiology. B, Biology
Yan-Qing WangBo-Ping Tang

Abstract

The nature of the interaction between human hemoglobin and C.I. acid red 27 was investigated systematically by ultraviolet-vis absorbance, circular dichroism, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques at pH 7.40. The quenching mechanism, binding constants, and the number of binding sites were determined by the quenching of human hemoglobin fluorescence in presence of C.I. acid red 27. The results showed that the nature of the quenching was of static type and the process of binding acid red 27 on human hemoglobin was a spontaneous molecular interaction procedure. The electrostatic and hydrophobic interactions played a major role in stabilizing the complex; The distance r between donor and acceptor was obtained to be 4.40 nm according to Förster's theory; The effect of acid red 27 on the conformation of human hemoglobin was analyzed using synchronous fluorescence, circular dichroism and three-dimensional fluorescence spectra.

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Citations

Feb 4, 2014·Journal of Photochemistry and Photobiology. B, Biology·Krishnamoorthy ShanmugarajMalaichamy Ilanchelian
May 30, 2012·Journal of Photochemistry and Photobiology. B, Biology·Yan-Qing Wang, Hong-Mei Zhang
Jul 28, 2016·Journal of Hazardous Materials·Anirban Basu, Gopinatha Suresh Kumar
Oct 22, 2016·Journal of Photochemistry and Photobiology. B, Biology·Anirban Basu, Gopinatha Suresh Kumar
Jan 18, 2017·The Journal of Physical Chemistry. B·Anirban Basu, Gopinatha Suresh Kumar
Jun 27, 2017·Molecular BioSystems·Anirban Basu, Gopinatha Suresh Kumar
Dec 14, 2011·Molecular Biology Reports·Hong-Mei ZhangYan-Qing Wang

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