The interaction of disagregin with the platelet fibrinogen receptor, glycoprotein IIb-IIIa

Biochemical and Biophysical Research Communications
J Karczewski, T M Connolly

Abstract

Disagregin, a 6 kDa protein isolated from salivary glands of the tick Ornithodoros Moubata, is a potent and selective inhibitor of fibrinogen dependent platelet aggregation and of the adhesion of platelets to fibrinogen (Karczewski et al. (1994) J. Biol. Chem. 269, 6702-6708). In the current study the interaction of disagregin with purified glycoprotein IIb-IIIa (GPIIb-IIIa) was examined. Biotin-labeled disagregin (b-disagregin) bound to GPIIb-IIIa immobilized on the surface of the ELISA plate. This binding was specific, dependent on divalent cations, and was blocked by the peptides fibrinogen gamma-chain fg gamma (400-411), GPIIb(296-306) and by the RGD-containing peptide, GRGDSP. Disagregin also bound to soluble GPIIb-IIIa as demonstrated in studies using the chemical crosslinker, BS3. This binding was inhibited by the peptides fg gamma (400-411) and GPIIb(296-306). In contrast to the results in the solid phase, peptide GRGDSP had no effect on the binding of b-disagregin to soluble GPIIb-IIIa. These data demonstrate that disagregin binds to GPIIb-IIIa through a mechanism distinct from that used by RGD-containing disintegrins. Further analysis of the region(s) of disagregin which bind to GPIIb-IIIa should provide useful inform...Continue Reading

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Citations

Nov 24, 2004·Biochimie·Sergio Daishi SasakiAparecida Sadae Tanaka
Dec 19, 2006·Insect Biochemistry and Molecular Biology·Francisco J Alarcon-ChaidezStephen K Wikel
Dec 12, 2012·International Journal for Parasitology·Elizabeth LouwChristine Maritz-Olivier
Jul 21, 2004·Folia Microbiologica·L Kovár
Jan 8, 2009·Cardiovascular Research·María P Ruiz-TorresManuel Rodríguez-Puyol
Mar 18, 2006·The Biochemical Journal·Eric FaudryAntonio R L Teixeira
Jul 11, 2012·Toxins·Teresa C F AssumpcaoIvo M B Francischetti
Jul 13, 2019·Frontiers in Physiology·Jindřich ChmelařMichail Kotsyfakis
Jan 23, 2021·International Journal of Molecular Sciences·Mohamed Amine JmelMichalis Kotsyfakis
Feb 5, 2021·Research and Practice in Thrombosis and Haemostasis·Danique L van den KerkhofIngrid Dijkgraaf
Aug 28, 2003·Archives of Biochemistry and Biophysics·Simone Sant'Anna AzzoliniAparecida Sadae Tanaka
Jan 16, 2004·Insect Biochemistry and Molecular Biology·Ben J Mans, Albert W H Neitz

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