Aug 18, 1977

The interaction of Hoechst 33258 and BrdU substituted DNA in the formation of sister chromatid exchanges

D G Stetka, A V Carrano


Sister chromatid exchanges (SCEs) are induced in cultured Chinese hamster cells by treatment with 5-bromodeoxyuridine (BrdU) or with Hoechst 33258 (H33258) plus BrdU. The SCE frequencies depend upon the number of H33258 molecules available per cell (or per base pair) and the number of brdU molecules available per cell, and not solely upon molarity. In addition, H33258 and BrdU act synergistically to induce SCEs. At low BrdU concentrations H 33258 induces very few SCEs. At high BrdU concentrations and similar concentrations of H33258, however, SCE frequencies are significantly increased. SCE frequencies decrease with time in successively harvested cells because of the depletion of H33258 from the medium due to DNA binding.

  • References21
  • Citations48


  • References21
  • Citations48


Mentioned in this Paper

Chinese Hamster
Sister Chromatid Exchange
DNA Binding
Base Pairing
Drug Augmentation
Dose-Response Relationship, Drug

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