The interaction of human serum albumin with selected lanthanide and actinide ions: Binding affinities, protein unfolding and conformational changes

Biochimie
Manjoor AliBadri N Pandey

Abstract

Human serum albumin (HSA), the most abundant soluble protein in blood plays critical roles in transportation of biomolecules and maintenance of osmotic pressure. In view of increasing applications of lanthanides- and actinides-based materials in nuclear energy, space, industries and medical applications, the risk of exposure with these metal ions is a growing concern for human health. In present study, binding interaction of actinides/lanthanides [thorium: Th(IV), uranium: U(VI), lanthanum: La(III), cerium: Ce(III) and (IV)] with HSA and its structural consequences have been investigated. Ultraviolet-visible, Fourier transform-infrared, Raman, Fluorescence and Circular dichroism spectroscopic techniques were applied to study the site of metal ions interaction, binding affinity determination and the effect of metal ions on protein unfolding and HSA conformation. Results showed that these metal ions interacted with carbonyl (CO..:)/amide(N..-H) groups and induced exposure of aromatic residues of HSA. The fluorescence analysis indicated that the actinide binding altered the microenvironment around Trp214 in the subdomain IIA. Binding affinity of U(VI) to HSA was slightly higher than that of Th(IV). Actinides and Ce(IV) altered the...Continue Reading

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Citations

May 28, 2019·Journal of Biomolecular Structure & Dynamics·Zahra Aramesh-BoroujeniMeissam Noroozifar
Jan 4, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rakhee YadavAmit Kumar
Feb 22, 2017·Journal of Clinical Laboratory Analysis·Rafał KuziołaKrzysztof Marczewski
Aug 13, 2017·Journal of Inorganic Biochemistry·Claudia WilkeAtsushi Ikeda-Ohno
Jun 3, 2020·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Zahra Aramesh-BoroujeniMeissam Noroozifar
Sep 13, 2018·Chemical Research in Toxicology·Astrid BarkleitAtsushi Ikeda-Ohno

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