The Interaction of Klebsiella pneumoniae With Lipid Rafts-Associated Cholesterol Increases Macrophage-Mediated Phagocytosis Due to Down Regulation of the Capsule Polysaccharide

Frontiers in Cellular and Infection Microbiology
Miguel A AresMiguel A De la Cruz

Abstract

Klebsiella pneumoniae successfully colonizes host tissues by recognizing and interacting with cholesterol present on membrane-associated lipid rafts. In this study, we evaluated the role of cholesterol in the expression of capsule polysaccharide genes of K. pneumoniae and its implication in resistance to phagocytosis. Our data revealed that exogenous cholesterol added to K. pneumoniae increases macrophage-mediated phagocytosis. To explain this event, the expression of capsular galF, wzi, and manC genes was determined in the presence of cholesterol. Down-regulation of these capsular genes occurred leading to increased susceptibility to phagocytosis by macrophages. In contrast, depletion of cholesterol from macrophage membranes led to enhanced expression of galF, wzi, and manC genes and to capsule production resulting in resistance to macrophage-mediated phagocytosis. Cholesterol-mediated repression of capsular genes was dependent on the RcsA and H-NS global regulators. Finally, cholesterol also down-regulated the expression of genes responsible for LPS core oligosaccharides production and OMPs. Our results suggest that cholesterol plays an important role for the host by reducing the anti-phagocytic properties of the K. pneumonia...Continue Reading

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Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·L Ponoop Prasad Patro, Thenmalarchelvi Rathinavelan
Apr 6, 2021·Frontiers in Cellular Neuroscience·Rebecca M Fleeman, Elizabeth A Proctor
Jul 8, 2021·Applied and Environmental Microbiology·Félix BornierXavier Charpentier
Jul 25, 2021·International Journal of Molecular Sciences·Stanislav Kotlyarov, Anna Kotlyarova

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